The aim of the present study was to compare one-step method to EDC/NHS crosslinking(EDC/NHS group)and one-step simultaneous method to EDC/NHS crosslinking and heparin immobilization(EDC/NHS-Heparin group)in improving physiochemical and biological properties of native collagen sponge(Control group).Modified collagen sponge overcome the disad-vantages of native collagen sponge.IR spectra suggest the change of the functional groups.DSC data indicate that the stability of caloric transformation in EDC/NHS group is slightly higher than that of EDC/NHS-Heparin group.The crosslinking degree,stability against enzymes,stability in morpho-logically and biomechanical properties of EDC/NHS-Heparin group are higher than those of EDC/NHS group,whereas,the water-binding capacity in EDC/NHS-Heparin group is lower than that of EDC/NHS group.HUVECs in EDC/NHS-Heparin group scaffold proliferate fast,migrate well and distribute uniformly.One-step simultaneous method gains the better effects in above aspects, heparinized collagen matrices increase in angiogenic potential and suit for defect repairing and tissue engineering.
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