医用植入物的表面改性是改善细胞行为、提高植入体骨整合的一种有效方法。通过激光加工及多重酸蚀在钛合金表面制备出微纳复合结构。采用 SEM 对钛合表面形貌进行表征。通过对不同表面形貌的植入体进行体外细胞培养，观察羟基磷灰石的形成、细胞形态及细胞粘附，分析不同表面形貌的生物活性。结果表明：微米结构表面能够促进成骨细胞粘附与铺展；与微米结构表面相比，微纳复合结构表面更有利于细胞粘附与伸展。微纳复合结构能够提高钛合金植入体的生物活性及骨整合能力。
Surface modification of medical implants was considered as an effective method to improve the cellular behaviors and the integration of tissue onto materials. The micro-nanostructured surface on the titanium alloy was prepared by laser treatment and multiple acid etching. The surface morphologies of different titanium alloy substrates were characterized by scanning electron microscopy (SEM). The effects of micro-nanostructured surfaces on the cellular responses were investigated in vitro by observing hydroxyapatite formation, cell morphology and cell adhesion. The results indicate that the micro-sized structure promoted the adhesion and proliferation of cultured osteoblasts. Furthermore, the micro-nanostructured surface was more conducive to cell adhension stretching compared with the micro-structured surface. All results suggest that the micro-nanostructured surface improved the biocompatibility and integration of tissue onto titanium alloy implants.
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