在培养浸矿微生物过程中,培养基中的铁含量不断减少,一般认为主要是生成了黄钾铁矾沉淀与胞外多聚物.对三种嗜酸浸矿菌Ferroplasma thermophilum,Leptospirillum ferriphilum和Acidithioobacillus ferrooxidans进行培养,发现他们能在嗜酸环境下生存,而且平均每个细胞生成超过10个纳米颗粒.通过分析纳米颗粒的形态与成分,发现颗粒中含有铁,而且纳米颗粒的产量很高.结果表明,在纯培养过程中减少的铁不仅参与生成黄钾铁矾,同时也被吸收进入细胞,合成含铁的纳米颗粒.
Jarosite and extracellular polymer substance generated during pure culture and bioleaching process have been widely accepted the main transformation of decreasing iron in the medium. In the present work, acidophilus bioleaching organisms Ferroplasma thermophilum, Leptospirillum ferriphilumand Acidithioobacillus ferrooxidans were cultured. It was found that they can live in low pH environment, and more than 10 particles in each cell intracellular nano-particles are synthesized in the cells. By analyzing the morphology and chemical composition of nano-particles, they were found to contain iron, and the three microorganisms belonged to high-yielding strains. The results show that the transformation of the decreasing iron ions is not only generating jarosite, but also taken into cells and synthesizing ferruginous nano-particles.
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