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研究了混合溶剂体系中阴离子分别为BF4-,PF6-,Cl-,Br-和r的14种离子液体对来源于黑布林糖苷酶催化合成红景天苷反应的影响.结果表明,在最佳反应条件下,β-糖苷酶的反应初速度和红景天苷的产率分别为3.3mmol/(L·h)和24.5%.离子液体咪唑阳离子的烷基链长(C2~C10)对β-糖苷酶的活性影响较大,当烷基链长为C6时,糖苷酶表现出较高的催化活性.

The preparation of salidroside was successfully carried out in fourteen ionic liquids (ILs)-containing systems using β-glycosidase from black plum seeds for the first time.The optimum conditions were determined for C6Mlm·BF4,pH,phosphate buffer content,and molar ratio of tyrosol to D-glucose to be 1% (v/v),5.9,20% (v/v),and 8∶1,under which the initial reaction rate and yield were 3.3 mmol/(L·h) and 24.5%,respectively.Moreover,the effects of 1-alkylimidazolium-based ILs possessing different alkyl chain lengths from C2 to C10 and a variety of anions including BF-4,PF-6,Cl-,Br-,and I- on enzyme activity in co-solvent systems were investigated.The results indicate that the optimal chain length of the alkyl substituent on the imidazolium ring of the cation was C6.

参考文献

[1] Hu X L;Lin S X;Yu D H;Qiu S F Zhang X Q Mei R H .[J].Cell Biology and Toxicology,2010,26:499.
[2] Qian EW;Ge DT;Kong SK .Salidroside promotes erythropoiesis and protects erythroblasts against oxidative stress by up-regulating glutathione peroxidase and thioredoxin.[J].Journal of Ethnopharmacology: An Interdisciplinary Journal Devoted to Bioscientific Research on Indigenous Drugs,2011(2):308-314.
[3] Akita H;Kurashima K;Nakamura T;Kato K .[J].Tetrahedron:Asymmetry,1999,10:2429.
[4] Wu SX;Zu YG;Wu M .High yield production of salidroside in the suspension culture of Rhodiola sachalinensis[J].Journal of Biotechnology,2003(1):33-43.
[5] 王梦亮,郭春侠.离子液体中β-葡萄糖苷酶生物催化合成红景天甙[J].催化学报,2011(06):1051-1055.
[6] Akita H;Kawahara E;Kishida M;Kato K .Synthesis of naturally occurring beta-D-glucopyranoside based on enzymatic beta-glycosidation[J].Journal of molecular catalysis, B. Enzymatic,2006(1/2):8-15.
[7] Yang R L;Li N;Zong M H .[J].Journal of Molecular Catalysis B:Enzymatic,2012,74:24.
[8] Lu WY;Lin GQ;Yu HL;Tong AM;Xu JH .Facile synthesis of alkyl beta-D-glucopyranosides from D-glucose and the corresponding alcohols using fruit seed meals[J].Journal of molecular catalysis, B. Enzymatic,2007(2):72-77.
[9] Tong A M;Lu W Y;Xu J H;Lin G Q .[J].Bioorganic and Medicinal Chemistry Letters,2004,14:2095.
[10] Yu HL;Xu JH;Lu WY;Lin GQ .Environmentally benign synthesis of natural glycosides using apple seed meal as green and robust biocatalyst[J].Journal of Biotechnology,2008(4):469-477.
[11] Tong A M;Xu J H;Lu W Y;Lin G Q .[J].Journal of Molecular Catalysis B:Enzymatic,2005,32:83.
[12] Lau R M;Van Rantwijk F;Seddon K R;Sheldon R A .[J].Organic Letters,2000,2:4189.
[13] Zhang Q H;Zhang S G;Deng Y Q .[J].Green Chemistry,2011,13:2619.
[14] van Rantwijk F;Sheldon R A .[J].Chemical Reviews,2007,107:2757.
[15] Kaftzik N;Wasserscheid P;Kragl U .[J].Organic Process Research & Development,2002,6:553.
[16] Zhao H;Jackson L;Song ZY;Olubajo A .Using ionic liquid [EMIM][CH3COO] as an enzyme-'friendly' co-solvent for resolution of amino acids[J].Tetrahedron, Asymmetry: The International Journal for Repid Publication on all Aspects of Asymmetry in Orgainc, Inorganic, Organometallic, Physical and Bio-Organic Chemistry,2006(17):2491-2498.
[17] Gorke J;Srienc F;Kazlauskas R .[J].Biotechnology and Bioprocess Engineering,2010,15:40.
[18] Zhao H .[J].Journal of Molecular Catalysis B:Enzymatic,2005,37:16.
[19] 王琴琴,郁蕙蕾,赵娜,李春秀,尚亚卓,刘洪来,许建和.利用交联杏仁粉作为廉价而稳定的催化剂通过优化逆水解反应体系显著提高长链烷基糖苷的平衡得率[J].催化学报,2012(02):275-280.
[20] 汪薇,宗敏华,娄文勇.不同反应体系中(S)-1-(4-甲氧基)-苯基乙醇的不对称生物合成[J].催化学报,2011(06):1003-1010.
[21] Lou W Y;Chen L;Zhang B B;Smith T Zong M H .[J].BMC Biotechnology,2009,9:90.
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