分别以细胞外实验和和细胞内实验来观察功能化氧化石墨烯( GO)对急性早幼粒细胞白血病细胞株NB4细胞的检测情况。通过比较不同探针浓度、温度、孵育时间等因素,探索最佳条件。结果表明,在室温下, GO吸附200 nmol/L单链DNA荧光探针,并淬灭其荧光；当与含靶分子( PML/RARα融合基因)的NB4细胞共孵育1小时后,探针便脱离GO,荧光得以恢复,从而准确检测出NB4细胞。
Developing a simple and costˉeffective strategy to diagnose and treat cancer with a single and minimum dose through noninvasive strategies is highly challenging. Nanoˉsized graphene and graphene oxide ( GO) are promising for biomedical applicaˉ tions, such as drug delivery and the photo thermal therapy of cancer. An acute promyelocytic leukemia line, such as NB4 cells, was detected by modified GO in extracellular and intracellular experiments. Results shows that GO can quench the fluorescence of the ssˉ DNA fluorescent probe and its fluorescence is restored after a PML/RARα fusion gene of NB4 cell lines is added. Therefore, the fusion gene can be detected accurately with this phenomenon. The best detection conditions for the fusion gene are found with assDˉ NA fluorescent probe concentration of 200 nmol/L in the presence of 0. 04 mg/L GO at room temperature for 1 h.
参考文献
| [1] | Xiaoying Yang;Xiaoyan Zhang;Yanfeng Ma .Superparamagnetic graphene oxide-Fe3O4 nanoparticles hybrid for controlled targeted drug carriers[J].Journal of Materials Chemistry: An Interdisciplinary Journal dealing with Synthesis, Structures, Properties and Applications of Materials, Particulary Those Associated with Advanced Technology,2009(18):2710-2714. |
| [2] | XU Pengˉcheng;LI Xinˉxin;YU Haiˉtao et al.Advanced nanˉ oporous materials for microˉgravimetric sensing to traceˉlevel bio/chemical molecules[J].Sensors(Basel),2014,14(10):19023-19056. |
| [3] | Xiaoming Sun;Zhuang Liu;Kevin Welsher .Nanoˉgraphene oxˉ ide for cellular imaging and drug delivery[J].Nano Research,2008,1:203-212. |
| [4] | 王晓丹,周宁琳,汪炜燕,汤毅达,章峻,沈健.镧修饰羧基化氧石墨烯的抑菌性能[J].新型炭材料,2012(05):385-392. |
| [5] | Novoselov K S;Geim A K;Morozov S V et al.Electric field effect in atomically thin carbon Films[J].SCIENCE,2004,306:666-669. |
| [6] | A Graphene Nanoprobe for Rapid, Sensitive, and Multicolor Fluorescent DNA Analysis[J].Advanced functional materials,2010(3):453. |
| [7] | Marissa Wu;Ravindra Kempaiah;PoˉJung Jimmy Huang.Adsorption and desorption of DNA on graphene oxide studied by fluorescently labeled oligonucleotides[J].LANGMUIR,2011:27,2731-2738. |
| [8] | Lu, CH;Zhu, CL;Li, J;Liu, JJ;Chen, X;Yang, HH .Using graphene to protect DNA from cleavage during cellular delivery[J].Chemical communications,2010(18):3116-3118. |
| [9] | Wang, Y.;Li, Z.;Hu, D.;Lin, C.-T.;Li, J.;Lin, Y. .Aptamer/graphene oxide nanocomplex for in situ molecular probing in living cells[J].Journal of the American Chemical Society,2010(27):9274-9276. |
| [10] | Lihua Wang;Kan-Yi Pu;Jing Li;Xiaoying Qi;Hai Li;Hua Zhang;Chunhai Fan;Bin Liu .A Graphene-Conjugated Oligomer Hybrid Probe for Light-Up Sensing of Lectin and Escherichia Coli[J].Advanced Materials,2011(38):4386-4391. |
| [11] | Nathaniel L Rosi;Chad A.Mirkin .Nanostructures in Biodiagnostics[J].Chemical Reviews,2005(4):1547-1562. |
| [12] | Daniel R. Dreyer;Sungjin Park;Christopher W. Bielawski .The chemistry of graphene oxide[J].Chemical Society Reviews,2010(1):228-240. |
| [13] | Haibo Wang;Qing Zhang;Xia Chu .Graphene Oxide-Peptide Conjugate as an Intracellular Protease Sensor for Caspase-3 Activation Imaging in Live Cells[J].Angewandte Chemie,2011(31):7065-7069. |
| [14] | He Shen;Min Liu;Hubrin He .PEGylated Graphene Oxide-Mediated Protein Delivery for Cell Function Regulation[J].ACS applied materials & interfaces,2012(11):6317-6323. |
| [15] | Lu, CH;Zhu, CL;Li, J;Liu, JJ;Chen, X;Yang, HH .Using graphene to protect DNA from cleavage during cellular delivery[J].Chemical communications,2010(18):3116-3118. |
| [16] | Li R;Tan Y H;Chen X H et al.Detection of PML/RARa fuˉ sion gene with functionalized graphene oxide[J].INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2013,14:12863-12872. |
| [17] | Tan,Y.;Bian,S.;Xu,Z.;Chen,X.;Qi,X.;Ren,F.;Li,L.;Guo,H.;Xu,A.;Zhang,L.;Wang,H. .The short isoform of the long-type PML-RARA fusion gene in acute promyelocytic leukaemia lacks sensitivity to all-trans-retinoic acid[J].British Journal of Haematology,2013(1):93-97. |
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