Enoxacin-loaded Poly (lactic-co-glycolic acid) Coating on Porous Magnesium Scaffold as a Drug Delivery System: Antibacterial Properties and Inhibition of Osteoclastic Bone Resorption

材料科学技术（英文）, 2016, 32(9): 865-873. doi: 10.1016/j.jmst.2016.07.013

Enoxacin-loaded Poly (lactic-co-glycolic acid) Coating on Porous Magnesium Scaffold as a Drug Delivery System: Antibacterial Properties and Inhibition of Osteoclastic Bone Resorption

Li Yang ^1, , Liu Xuqiang ^2, , Tan Lili ^3, , Ren Ling ^3, , Wan Peng ^3, , Hao Yongqiang ^1, , Qu Xinhua ^1,*,, , Yang Ke ^3,*,, , Dai Kerong ^1,*,,

1. Shanghai Key Laboratory of Orthopaedic Implants, Department of Orthopaedic Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China

2. Department of Orthopedics, First Affiliated Hospital of Nanchang University, Artificial Joint Engineering and Technology Research Center of Jiangxi Province, Nanchang 330006, China

3. Institute of Metal Research, Chinese Academy of Sciences, Shenyang 110016, China

Correspondence author: Qu Xinhua, xinhua_qu@126.comCorrespondence author: Yang Ke, kyang@imr.ac.cnCorrespondence author: Dai Kerong, krdai@163.com

Keywords： Porous magnesium scaffold , Poly (lactic-co-glycolic acid) (PLGA) , Drug delivery system , Bactericidal activity , Enoxacin , Osteolysis

Implant-associated infection remains a difficult medical problem in orthopedic surgery. Therefore, the development of multifunctional bone implants for treating infection and regenerating lost bone tissue, which may be a result of infection, is important. In the present study, we report the fabrication of enoxacin-loaded poly (lactic-co-glycolic acid) (PLGA) coating on porous magnesium scaffold (Enox-PLGA-Mg) which combine the favorable properties of magnesium, the antibacterial property and the effect of inhibition of osteoclastic bone resorption of enoxacin. The drug loaded PLGA coating of Mg scaffold enables higher drug loading efficiency (52%-56%) than non-coating enoxacin loaded Mg scaffold (Enox-Mg) (4%-5%). Enox-PLGA-Mg exhibits sustained drug release for more than 14 days, and this controlled release of enoxacin significantly inhibits bacterial adhesion and prevented biofilm formation by Staphylococcus epidermidis (ATCC35984) and Staphylococcus aureus (ATCC25923). Biocompatibility tests with Balb/c mouse embryo fibroblasts (Balb/c 3T3 cells) indicate that PLGA-Mg has better biocompatibility than Mg. Finally, we also demonstrate that Enox-PLGA-Mg extract potently inhibited osteoclast formation in vitro. Therefore, Enox-PLGA-Mg has the potential to be used as a multifunctional controlled drug delivery system bone scaffolds to prevent and/or treat orthopedic peri-implant infections.

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Enoxacin-loaded Poly (lactic-co-glycolic acid) Coating on Porous Magnesium Scaffold as a Drug Delivery System: Antibacterial Properties and Inhibition of Osteoclastic Bone Resorption

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