重离子诱导细胞衰老研究

原子核物理评论 , 2014, (2): 201-209. doi: 10.11804/NuclPhysRev.31.02.201

重离子诱导细胞衰老研究

1.中国科学院近代物理研究所，兰州 730000; 中国科学院重离子束辐射生物医学重点实验室，兰州 730000; 中国科学院大学，北京 100049

2.中国科学院近代物理研究所,兰州,730000

3.中国科学院近代物理研究所,兰州,730000

4.中国科学院近代物理研究所,兰州,730000

基金项目: National Natural Science Foundation of China(U1232125) 国家重点基础研究发展计划项目(973计划)(2010CB834201)；国家自然科学基金资助项目(U1232125)@@@@National Basic Research Program of China(973 Program)(2010CB834201)

关键词：细胞衰老 , 肿瘤发生 , DNA团簇损伤 , 碳离子束

Research on Cellular Senescence Induced by Heavy Ion

Keywords： cellular senescence , tumorigenesis , clustered DNA damage , carbon ion beam

综述了细胞衰老诱导因素和衰老细胞的特征，提出细胞衰老在癌症发生发展过程中具有双重作用效应。同时，以X射线和12 C6+离子束对黑色素瘤细胞系92-1或人正常成纤维细胞系MRC5进行辐照，观察分析DNA损伤应答效应和细胞衰老诱导。实验结果表明，相对于X射线，12C6+离子束能导致DNA团簇损伤，持续激活DNA损伤应答，更容易诱导细胞衰老；12 C6+离子束能同时诱导癌细胞和正常细胞衰老。这些实验结果暗示开展12 C6+离子束诱导细胞衰老研究具有紧迫性。

Cellular senescence played double roles in the genesis and development of cancer, which was put forward through reviewing the cases and characteristics of cellular senescence. In addition, human uveal melanoma cells and human fetal lung fibroblast (MRC5) cells were irradiated by X-rays or 12C6+ion beam. DNA damage response (DDR) and cellular senescence were analyzed. The results showed that DDR was activated persistently by the unrepairable clustered DNA damage, which indicated that inducing same ratios of cellular senescence needed lower doses of 12C6+ ion beam than X-rays. Furthermore, both 92-1 cells and MRC5 cells treated by carbon ion beam underwent cellular senescence. These results pointed out the urgency to investigate the mechanism of cellular senescence induced by 12C6+ ion beam.

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