中药物质基础复杂,对其活性成分的分离一直是中药研究的难题。基于高压制备液相的多维色谱系统在高压制备液相色谱的基础上,结合了多种分离技术,极大地提高了色谱系统的分离性能和分离效率,更有利于对物质基础复杂的中药样品进行分离纯化。本文介绍了基于高压制备液相系统的多维色谱系统的基本原理、分离模式以及关键技术,并综述了其在中药分离纯化中的应用。
Separation of the active ingredients of Chinese medicine is always difficult due to its complexity matrix. Recent years, the preparative-high performance liquid chromatography (Pre-HPLC)-based multidimensional chromatography combined with the characteristics of different separation technology improved the separation capability and efficiency, and was applied to the separation and purification of complex traditional Chinese medicine (TCM). In this review, the based principle, separation mode and key technology of preparative-HPLC-based multidimensional chromatography, as well as its application in TCM research were introduced.
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