基于阳离子型共轭聚合物和核酸适配体的高灵敏铅离子快速检测方法

分析化学, 2016, 44(7): 1092-1098. doi: 10.11895/j.issn.0253-3820.160010

基于阳离子型共轭聚合物和核酸适配体的高灵敏铅离子快速检测方法

刘兴奋 ^1,*,, , 王亚腾 ^1, , 华笑笑 ^1, , 黄艳琴 ^1, , 冯晓苗 ^1, , 范曲立 ^1, , 黄维 ^1,2,

1.南京邮电大学信息材料与纳米技术研究院, 有机电子与信息显示国家重点实验室培育基地, 江苏省有机电子和信息显示协同创新中心, 南京 210023

2.南京工业大学先进材料研究院, 江苏省柔性电子重点实验室, 先进生物与化学制造协同创新中心, 南京 211816

通讯作者: 刘兴奋, iamxfliu@njupt.edu.cn

录用日期: 2016-04-19

关键词：阳离子型共轭聚合物 , 核酸适配体 , 铅离子 , 荧光共振能量转移

Rapid Detection of Lead Ion (Ⅱ) Based on Cationic Conjugated Polymer and Aptamer

Xing-Fen LIU ^1,*,, , Ya-Teng WANG ^1, , Xiao-Xiao HUA ^1, , Yan-Qin HUANG ^1, , Xiao-Miao FENG ^1, , Qu-Li FAN ^1, , Wei HUANG ^1,2,

1.Key Laboratory for Organic Electronics & Information Displays and Institute of Advanced Materials, Jiangsu National Synergetic Innovation Center for Advanced Materials, Nanjing University of Posts and Telecommunications, Nanjing 210023, China

2.Key Laboratory of Flexible Electronics & Institute of Advanced Materials, Jiangsu National Synergetic Innovation Center for Advanced Materials, Nanjing Tech University (Nanjing Tech), Nanjing 211816, China

Correspondence author: Xing-Fen LIU, iamxfliu@njupt.edu.cn

Keywords： Cationic conjugated polymer , Aptamer , Lead ion (Ⅱ) , Fluorescence resonance energy transfer

建立了基于主链含芴的阳离子型对芳撑乙炔衍生物(poly{[9,9-bis(6'-(N,N,N-diethylmethyl ammonium)hexyl)-2,7-fluorenyleneethynylene]-alt-co-[2,5-bis(3'-(N,N,N-diethylmethylammonium)-1'-oxapropyl)-1,4-phenylene]tetraiodide}, PFEP)和核酸适配体快速检测Pb²⁺的新方法。使用选择性更高的核酸适配体序列,有效降低了Hg²⁺对Pb²⁺检测的干扰,提高了检测的选择性和灵敏度。用于识别Pb²⁺的核酸探针(TBAA)末端用荧光素标记(5'-6-FAM-GGAAGGTGTGGAAGG-3')。当其与Pb²⁺发生特异性结合时,形成电荷密度高于单链DNA的G-四链体结构,与PFEP之间的静电作用增强,使能量供体(聚合物)与受体(荧光素)之间的距离拉近,发生更高效的荧光共振能量转移(Fluorescence resonance energy transfer, FRET)。其它金属离子由于不能和TBAA结合,且金属离子本身对聚合物和荧光素的荧光有一定程度的猝灭,因此其FRET信号远低于空白对照。本方法快速、灵敏,几分钟内即可完成检测,常见金属离子均不干扰检测。对湖水中Pb²⁺的检测限为1 nmol/L,远低于饮用水国家标准中对Pb²⁺浓度的限量标准。本方法为水中Pb²⁺的检测提供了一种简便、快速、高效的新方法。

A sensitive method for Pb²⁺ detection based on a cationic conjugated polymer and aptamer was established. By selecting a more specific aptamer probe, the interference of Pb²⁺ detection from Hg²⁺ was eliminated remarkably. The probe for Pb²⁺ recognition and combination is a single stranded oligonucleotide labeled with fluorescein (TBAA, 5'-6-FAM-GGAAGGTGTGGAAGG-3'). When combining with Pb²⁺ with high specificity, the random coiled probe changed to a G-quadruplex with higher charge density, which enhanced the electrostatic interactions between the oligonucleotide and cationic conjugated polymer, thus the two fluorophores were in more close proximity, leading to a significantly increased fluorescence resonance energy transfer (FRET) signal. However, other non-target metal ions produced much lower FRET signals because they could not combine with the probe and thus quenched the fluorescence of the conjugated polymer and fluorescein. This method was rapid, highly specific and sensitive, and the common metal ions including Li⁺, Na⁺, K+, Ca²⁺, Mg²⁺, Ba²⁺, Fe²⁺, Co²⁺, Ni²⁺, Cu²⁺, Zn²⁺, Cd²⁺ and Hg²⁺ exhibited no influence on the detection of Pb²⁺. The limit of detection for Pb²⁺ in lake water was estimated to be 1 nmol/L (3σ), which was far below the maximum permitted level of Pb²⁺ in drink water regulated by the national standard for drinking water quality. Therefore, this FRET-based method provides a new simple, rapid, and efficient method for detection of Pb²⁺ in various source of water.

参考文献

[1]	LIU Zhong-Hui, WANG Feng-Shan.. Journal of Environment and Health, 2007,24(2):122-123 刘忠慧, 王凤山..环境与健康杂志,2007,24(2):122-123
[2]	SONG Di-Shen, HE Hai-Yan, HE Zhi-Jian, CUI Shu-Ya, DU Jun-Liang.. Chinese Journal of Analysis Laboratory, 2015,34(8):944-946 宋迪燊, 何海艳, 何志坚, 崔书亚, 杜军良..分析试验室,2015,34(8):944-946
[3]	WANG Xiao-Hui, ZHANG Yu-Ling, LIU Na, WANG Lin.. Chinese Journal of Spectroscopy Laboratory, 2008,25(6):1183-1187 王晓晖, 张玉玲, 刘娜, 王琳..光谱实验室,2008,25(6):1183-1187
[4]	Tang S, Tong P, Li H, Tang J, Zhang L.. Biosens^ Bioelectron^, 2013,42:608-611
[5]	Liu C W, Huang C C, Chang H T.. Anal^ Chem^, 2009,81(6):2383-2387
[6]	Song W, Zhang Q, Xie X, Zhang S.. Biosens^ Bioelectron^, 2014,61:51-56
[7]	Pavlov V, Xiao Y, Shlyahovsky B, Willner I.. J^ Am^ Chem^ Soc^, 2004,126(38):11768-11769
[8]	Liang H R, Hu G Q, Xue X H, Li L, Zheng X X, Gao Y W, Yang S T, Xia X Z.. Virus Res^, 2014,184:7-13
[9]	Kim Y S, Song M Y, Jurng J, Kim B C.. Anal^ Biochem^, 2013,436(1):22-28
[10]	Cao H Y, Yuan A H, Shi X S, Chen W, Miao Y.. Oncol^ Rep^, 2014,32(5):2054-2060
[11]	WEI Xiao-Ping, YANG Feng, DING Fan, LI Jian-Ping.. Chinese J^ Anal^ Chem^, 2014,42(7):942-947 魏小平, 杨峰, 丁璠, 李建平..分析化学,2014,42(7):942-947
[12]	Nguygen B L, Jeong J E, Jung I H, Kim B, Le V S, Kim I, Kyhm K, Woo H Y.. Adv^ Funct^ Mater^, 2014,24(12):1748-1757
[13]	Neves Miguel A D, Blaszykowski C, Thompson M.. Anal^ Chem^, 2016,88(6):3098-3106
[14]	LIU Zhi-Wei, TONG Zhao-Yang, HAO Lan-Qun, LIU Bing, MU Xi-Hui, ZHANG Jin-Ping, GAO Chuan.. Chinese J^ Anal^ Chem^, 2014,42(8):1143-1148
[15]	刘志伟, 童朝阳, 郝兰群, 刘冰, 穆晞惠, 张金平, 高川..分析化学,2014,42(8):1143-1148 LI Xia, TAN Hui, LI Yun-Lei, WU Zhao-Yang, SHEN Guo-Li, YU Ru-Qin.. Chinese J^ Anal^ Chem^, 2014,42(5):629-635
[16]	李霞, 谭慧, 李云磊, 吴朝阳, 沈国励, 俞汝勤..分析化学,2014,42(5):629-635 Liu X F, Shi L, Hua X X, Huang Y Q, Su S, Fan Q L, Wang L H, Huang W.. ACS Appl^ Mater^ Inter^, 2014,6(5):3406-3412
[17]	ZHANG Song-Bai, ZHENG Li-Ying, HU Xia, SHEN Guang-Yu, LIU Xue-Wen, SHEN Guo-Li, YU Ru-Qin.. Chinese J^ Anal^ Chem^, 2015,43(11):1688-1694
[18]	张松柏, 郑丽英, 胡霞, 沈广宇, 刘学文, 沈国励, 俞汝勤..分析化学,2015,43(11):1688-1694 Kim Y S, Chung J, Song M Y, Jurng J, Kim B C.. Biosens^ Bioelectron^, 2014,54:195-198
[19]	Qu L, Xu J, Tan X, Liu Z, Xu L, Peng R.. Acs Appl^ Mater^ Inter^, 2014,6(10):7309-7315
[20]	Satoru N, Takahiko N, Elzbieta G, Bernard J, Shigeori T.. Chembiochem, 2006,7(11):1730-1737
[21]	Zhang D, Yin L, Meng Z, Yu A, Guo L, Wang H.. Anal^ Chim^ Acta, 2014,812:161-167
[22]	Lu Y, Li X, Wang G, Tang W.. Biosens^ Bioelectron^, 2013,39(1):231-235
[23]	Li X, Wang G, Ding X, Chen Y, Gou Y, Lu Y.. Phys^ Chem^ Chem^ Phys^, 2013,15(31):12800-12804
[24]	Duan X, Liu L, Feng F, Wang S.. Accounts Chem^ Res^, 2010,43(2):260-270
[25]	Feng X, Liu L, Wang S, Zhu D.. Chem^ Soc^ Rev^, 2010,39(7):2411-2419
[26]	GB 5749-2006, Standards for drinking water quality^^ National Standards of the People's Republic of China生活饮用水卫生标准,中华人民共和国国家标准. GB 5749-2006
[27]	GB 5749-2006, Standards for drinking water quality^^ National Standards of the People's Republic of China
[28]	Huang YQ, Fan Q L, Zhang G W, Chen Y, Lu X M, Huang W.. Polymer, 2006,47(15):5233-5238
[29]	He F, Tang Y, Wang S, Li Y, Zhu D.. J^ Am^ Chem^ Soc^, 2005,127(35):12343-12346
[30]	Liu X F, Ouyang L, Huang Y, Feng X, Fan Q, Huang W.. Polym^ Chem^, 2012,3(3):703-709
[31]	Chen Y, Pu K Y, Fan Q L, Qi X Y, Huang Y Q, Lu X M, Huang W.. J^ Polym^ Sci^ Poly^ Chem^, 2009,47(19):5057-5067
[32]	Laurenti M, Blanco F G, Lopez-Cabarcos E, Rubio-Retama J.. Polym^ Int^, 2013,62(5):811-816
[33]	Chen Y, Fan Q L, Wang P, Zhang B, Huang Y Q, Zhang G W, Lu X M, Chan H S O, Huang W.. Polymer, 2006,47(15):5228-5232
[34]	Wu Y, Tan Y, Wu J, Chen S, Chen Y Z, Zhou X, Jiang Y, Tan C.. ACS Appl^ Mater^ Inter^, 2015,7(12):6882-6888
[35]	Saha S K, Ghosh K R, Gao J, Wang Z.. Macromol^ Rapid Comm^, 2014,35(18):1592-1954
[36]	Lee K M, Chen X, Fang W, Kim J M, Yoon J.. Macromol^ Rapid Comm^, 2011,32(6):497-500
[37]	Yu M, He F, Tang Y, Wang S, Li Y, Zhu D.. Macromol^ Rapid Comm^, 2007,28(12):1333-1338
[38]	Cheng S, Zheng B, Wang M Z, Ge X W, Zhao Q, Liu W, LAM M H W.. Biosens^ Bioelectron^, 2014,53:479-485

上一张下一张

计量

下载量()
访问量()

作者相关

文章评分

您的评分：
1

0%
2

0%
3

0%
4

0%
5

0%

材料期刊网