针对肿瘤标志物高灵敏度现场快速检测的需求,将电化学检测技术与电子技术相结合,采用差分脉冲伏安法(Differential pulse voltammetry, DPV)研制了一种可用于肿瘤标志物现场快速检测的便携式仪表,检测电压和电流的分辨率分别为0.8 mV和1 nA。结合实验室自制微流控纸芯片,利用该便携式仪表对肿瘤标志物癌胚抗原(Carcinoembryonic antigen,CEA)进行检测,实验结果表明,在1-500μg/L浓度范围内,DPV峰值电流响应与CEA抗原浓度对数呈线性关系,线性相关系数为0.998,检出限为10 pg/mL。根据抗原抗体特异性结合和电化学检测原理,检测到电化学反应的微弱电流后,仪表可以根据已经标定的电流与浓度之间的比例关系自动计算出肿瘤标志物的浓度。此便携式仪表具有检测灵敏度高、检出限低等优点,可广泛应用于肿瘤标志物的即时检测。
By combining electrochemical detection technique and electronic technique, a portable device which adopted differential pulse voltammetry (DPV) measurement was developed to meet the needs of high sensitivity and on-site rapid detection for tumor marker. The voltage and current resolution of portable device were 0.8 mV and 1 nA, respectively. Combined with laboratory made paper-based analytical device, carcinoembryonic antigen(CEA) was tested by portable device. Experimental results revealed that the DPV peak currents showed good linear relation with the logarithm of CEA concentration in the range from 1 μg/L to 500 μg/L. Its corresponding correlation coefficient was 0.998 and the limit of detection was 10 pg/mL. According to the principle of specific binding of antibody and antigen and electrochemical detection, CEA concentration could be calculated automatically according to the linear equation. The portable device had high sensitivity and low detection limit features which could be widely used in the point-of-care testing of tumor marker.
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