以2,6-二甲基吡啶-3,5-二甲酸二乙酯为起始原料,经N-溴代丁二酰亚胺(NBS)溴代、亲电取代反应合成了时间分辨荧光免疫分析双功能螯合剂2,6-{N,N',N,N'-[二(2,2'-联吡啶-6,6'-二甲基)]二(氨甲基)}-吡啶-3,5-二羧酸二乙酯.经差热分析仪(DTA)、傅里叶变换红外光谱仪(FTIR)、核磁共振波谱仪(1H NMR)、质谱仪(MS)等技术手段表征确认了化合物结构和性能.对该化合物与铕离子形成螯合物的荧光性质研究表明:激发光谱波长范围较宽,激发峰值为322 rn;荧光发射峰为597 nm(5D0-7F1)、618 nm(5D0-7F);荧光寿命为918μs;量子产率Φ=0.249.
A bifuntional cryptate chelate 2,6-{ N,N',N,N'-[bis (2,2'-bipyridine-6,6'-dimethyl)]bis (aminomethyl) }-pyridine-3,5-diethyl diester for homogeneous time-resolved fluorimmunoassay product was synthesized from the raw material diethyl 2,6-dimethylpyridine-3,5-dicarboxylate through N-bromobutanimide (NBS) bromination,electrophilic substitution.The structure and property were confirmed by differential thermal analysis(DTA) Fourier transform infrared spectroscopy (FTIR),proton nuclear magnetic resonance spectroscopy(1H NMR) and mass spectrometry(MS).Also,the spectral properties of the Eu(Ⅲ)-cryptate chelate,such as absorption spectra,emission spectra,and fluorescence lifetime,were discussed.The fluorescence spectroscopic properties of Eu(Ⅲ)-cryptate are as follows:the excitation peak is at 322 nm,the narrow emission spectrum peaks are at 597 nm (5D0-TF1) and 618 nm (5Do-7F2),respectively,the Stokes shift is 290 nm,the fluorescence lifetime is 918 μs,and the fluorescence quantum yield is Φ =0.249.
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