按文献方法合成了两种Dawson结构的多金属氧酸盐,并对其结构进行紫外光谱和红外光谱分析.以H6[P2Mo18O62]和H8[P2Mo17Cr(OH2)O61](简写为P2Mo18和P2Mo17Cr)为效应物,采用紫外分光光度法和酶动力学方法研究两种Dawson结构的多金属氧酸盐效应物对蘑菇酪氨酸酶二酚酶活性的抑制作用.结果表明,P2Mo18和P2Mo17Cr对酪氨酸酶二酚酶均具有显著的抑制效果,测定抑制酪氨酸酶活力下降5O％的抑制浓度(IC50)分别为(0.482±0.009) mmoL/L和(0.503±0.011) mmol/L.动力学分析表明,P2Mo18和P2Mo17 Cr对酪氨酸酶的抑制作用均表现为可逆的竞争型,抑制常数K1分别为0.212和0.249 mmol/L.其中,综合考虑IC50值和抑制常数,P2 Mo18对酪氨酸酶二酚酶活性的抑制效果略优于P2 Mo17 Cr.
参考文献
| [1] | Young-Hong Jo;Go-Un Seo;Hyun-Gyun Yuk;Seung-Cheol Lee.Antioxidant and tyrosinase inhibitory activities of methanol extracts from Magnolia denudata and Magnolia denudata var. purpurascens flowers[J].Food Research International,20122(2):197-200. |
| [2] | Si, Y.-X.;Wang, Z.-J.;Park, D.;Chung, H.Y.;Wang, S.-F.;Yan, L.;Yang, J.-M.;Qian, G.-Y.;Yin, S.-J.;Park, Y.-D..Effect of hesperetin on tyrosinase: Inhibition kinetics integrated computational simulation study[J].International Journal of Biological Macromolecules: Structure, Function and Interactions,20121(1):257-262. |
| [3] | Yue-Xiu Si;Sunyoung Ji;Nai-Yun Fang;Wei Wang;Jun-Mo Yang;Guo-Ying Qian;Yong-Doo Park;Jinhyuk Lee;Shang-Jun Yin.Effects of piperonylic acid on tyrosinase: Mixed-type inhibition kinetics and computational simulations[J].Process biochemistry,201311(11):1706-1714. |
| [4] | 裴超俊 .天麻素对酪氨酸酶的抑制作用及黑色素生成的影响[D].苏州大学,2013. |
| [5] | 杨美花;石艳;李智聪;王秋红;陈清西.原儿茶酸对小鼠黑色素瘤B16细胞酪氨酸酶活力及黑色素生成的抑制效应[J].厦门大学学报（自然科学版）,2013(6):842-845. |
| [6] | Lianzhu Lin;Yi Dong;Haifeng Zhao;Lingrong Wen;Bao Yang;Mourning Zhao.Comparative evaluation of rosmarinic acid, methyl rosmarinate and pedalitin isolated from Rabdosia serra (MAXIM.) HARA as inhibitors of tyrosinase and a-glucosidase[J].Food Chemistry,20113(3):884-889. |
| [7] | Yu, Zhi-Long;Zhang, Zeng;Zeng, Wei-Cai.Investigation of antibrowning activity of pine needle (Cedrus deodara) extract with fresh-cut apple slice model and identification of the primary active components[J].European food research and technology =: Zeitschrift fur Lebensmittel-Untersuchung und -Forschung. A,20144(4):669-678. |
| [8] | Ha YM;Kim JA;Park YJ;Park D;Kim JM;Chung KW;Lee EK;Park JY;Lee JY;Lee HJ;Yoon JH;Moon HR;Chung HY.Analogs of 5-(substituted benzylidene)hydantoin as inhibitors of tyrosinase and melanin formation.[J].Biochimica et Biophysica Acta. General Subjects,20116(6):612-619. |
| [9] | Qun Tang;Chun-Jing Zhang;Chun-Hua Zhang.Two sandwich-type compounds from Keggin polyoxometalate, d~(10) metals and the product of in situ catalyzed decarboxylation of pyrazine-2,3-dicarboxylic acid[J].Inorganic Chemistry Communications,2012:238-242. |
| [10] | 谭荣欣;崔术新;王慧;任玉兰.4种含钛的多金属氧酸盐的合成及抗肿瘤活性研究[J].分子科学学报,2013(2):152-155. |
| [11] | 甘强;刘霞;冯长根.多金属氧酸盐抗肿瘤研究进展[J].科技导报,2011(31):75-79. |
| [12] | 陈丙年;冯珍鸽;王力.含硅多金属氧酸盐的抑菌作用[J].高等学校化学学报,2011(5):1033-1036. |
| [13] | 冯珍鸽;蔡慧农;王力;杨秋明.含钒多金属氧酸盐的抑菌活性[J].应用化学,2010(8):990-992. |
| [14] | Lee, Sang-Yong;Fiene, Amelie;Li, Wenjin;Hanck, Theodor;Brylev, Konstantin A.;Fedorov, Vladimir E.;Lecka, Joanna;Haider, Ali;Pietzsch, Hans-Juergen;Zimmermann, Herbert;Sevigny, Jean;Kortz, Ulrich;Stephan, Holger;Mueller, Christa E..Polyoxometalates-Potent and selective ecto-nucleotidase inhibitors[J].Biochemical Pharmacology,20152(2):171-181. |
| [15] | 郑阿萍;王芳;邢蕊;蒋爱华;王力.两种多酸型酪氨酸酶抑制剂的性能研究[J].高等学校化学学报,2014(3):476-481. |
| [16] | 邢蕊;郑阿萍;王芳;王力.钒取代的多酸盐对酪氨酸酶抑制机理的研究[J].分子科学学报,2015(5):436-440. |
| [17] | Shubo Jing;Zhenlii Wang;Wanchun Zhu.OXIDATION OF CYCLOHEXANE WITH HYDROGEN PEROXIDE CATALYZED BY DAWSON-TYPE VANADIUM-SUBSTITUTED HETEROPOLYACIDS[J].Reaction kinetics & catalysis letters,20061(1):55-61. |
| [18] | Xie LP;Chen QX;Huang H;Liu XD;Chen HT;Zhang RQ.Inhibitory effects of cupferron on the monophenolase and diphenolase activity of mushroom tyrosinase.[J].The international journal of biochemistry & cell biology,200312(12):1658-1666. |
| [19] | 芶琳;卢航;王西瑶;刘晓倩.硫代硫酸钠对酪氨酸酶的抑制作用[J].厦门大学学报（自然科学版）,2014(2):263-266. |
| [20] | Yin SJ;Si YX;Chen YF;Qian GY;Lu ZR;Oh S;Lee J;Lee S;Yang JM;Lee DY;Park YD.Mixed-type inhibition of tyrosinase from Agaricus bisporus by terephthalic acid: computational simulations and kinetics.[J].The protein journal,20114(4):273-280. |
| [21] | 宋康康;邱凌;黄璜;陈清西.熊果甙作为化妆品添加剂对酪氨酸酶抑制作用[J].厦门大学学报(自然科学版),2003(6):791-794. |
| [22] | HU Xi-yu;CHEN Bing-nian;WANG Li;CHEN Fa-he.Inhibition of α/β-K6P2W18O62· 10H2O on the Activity of Mushroom Tyrosinase and Its Antimicrobial Effects[J].高等学校化学研究（英文版）,2012(5):862-865. |
| [23] | Yong-Hua Hu;Xuan Liu;Yu-Long Jia.Inhibitory kinetics of chlorocinnamic acids on mushroom tyrosinase[J].Journal of Bioscience and Bioengineering,20142(2):142-146. |
| [24] | Chai, W.-M.;Liu, X.;Hu, Y.-H.;Feng, H.-L.;Jia, Y.-L.;Guo, Y.-J.;Zhou, H.-T.;Chen, Q.-X..Antityrosinase and antimicrobial activities of furfuryl alcohol, furfural and furoic acid[J].International Journal of Biological Macromolecules: Structure, Function and Interactions,2013:151-155. |
| [25] | Shyi-Neng Lou;Ming-Wen Yu;Chi-Tang Ho.Tyrosinase inhibitory components of immature calamondin peel[J].Food Chemistry,20123(3):1091-1096. |
| [26] | Dogan S.;Turan P.;Dogan M.;Alkan M.;Arslan O..Inhibition kinetic and mechanism of polyphenol oxidase from various sources by diethyldithiocarbamic acid.[J].International Journal of Food Science and Technology,20087(7):1316-1322. |
上一张
下一张
上一张
下一张
计量
- 下载量()
- 访问量()
文章评分
- 您的评分：
-
10%
-
20%
-
30%
-
40%
-
50%