烟草花叶病毒(TMV)由于其良好的生物相容性、单分散性、多价性、低成本等优点,已作为功能材料的基本构筑单元应用于光电器件、组织工程、疫苗载体,无机纳米材料制备等研究领域. 然而,相比于棒状的TMV,球状TMV纳米颗粒无核酸分子,抗环境影响能力更强,比表面积更大. 本文利用蛋白质的热致变性原理,对经基因和化学改性后的棒状TMV如半胱氨酸突变体(TMV-Cys)、赖氨酸突变体(TMV-EPMK)和β-环糊精(β-CD)修饰的TMV(TMV-β-CD)进行热变性处理,探究其形成球型纳米颗粒(TMV-SNP)的能力及功能性. 结果显示,改性后的TMV经历热变性后可得到形貌均一的球型纳米颗粒,且其暴露在纳米颗粒表面的功能基团Cys、Lys和β-CD仍具有反应活性.
Tobacco mosaic virus(TMV) due to its good biocompatibility, monodisperse, multivalent, low cost and other advantages, has been used as the basic building blocks used in optoelectronic devices, tissue engineering, vaccine vector, inorganic material preparation fields.However, compared to the rod like TMV, spherical TMV nanoparticles with no nucleic acid molecules are more resistant to environmental impact and have a larger specific surface area.The genetic and chemical modification of TMV, such as cysteine mutants of TMV(TMV TMV-Cys), lysine mutant of TMV(TMV-EPMK) and β-cyclodextrin(β-CD) modified TMV(TMV-β-CD) were conducted by thermal denaturation, and we studied their formation process and functionality.The results shows that the modified TMV can transit into the uniform spherical nanoparticles, and the functional groups exposed to the surface of the nanoparticles still keep their reactive ability.
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