以块体SiO2大孔材料为基质,甲基丙烯酸缩水甘油酯(GMA)和甲基丙烯酸羟乙酯(HEMA)为功能单体,通过原位聚合和溶剂蒸发制备P(GMA-co-H EMA) /SiO2大孔复合材料,用SEM、EDS、BET、FTIR和TG-DTA对样品进行表征,并将其用于固定褶皱假丝酵母脂肪酶(CRL).结果表明:SiO2大孔材料很强的毛细管作用使共聚物均匀地涂敷在其孔壁上,形成P(GMA-co-HEMA)/SiO2复合纳米薄膜.共聚物的负载量和亲疏水性可分别通过改变单体浓度和体积比进行调控,当单体体积浓度为10％、GMA和HEMA的体积比为9∶1时大孔复合材料固定化脂肪酶比酶活达到最高,为3 886.9 U/g,与底物反应重复操作8批次后剩余酶活率为68.7％.
参考文献
| [1] | Valentin Valtchev.Preparation of regular macrophorous structures built of intergrown silicalite-1 nanocrystals[J].Journal of Materials Chemistry: An Interdisciplinary Journal dealing with Synthesis, Structures, Properties and Applications of Materials, Particulary Those Associated with Advanced Technology,20026(6):1914-1918. |
| [2] | Nam YS;Yoon JJ;Park TG.A novel fabrication method of macroporous biodegradable polymer scaffolds using gas foaming salt as a porogen additive.[J].Journal of biomedical materials research, Part B. Applied biomaterials,20001(1):1-7. |
| [3] | Patrick Adlercreutz.Immobilisation and application of lipases in organic media[J].Chemical Society Reviews,201315(15):6406-6436. |
| [4] | Junghun Suh.Synthetic Artificial Peptidases and Nucleases Using Macromolecular Catalytic Systems[J].Accounts of Chemical Research,20037(7):562-570. |
| [5] | Andrew C. Spowage;Andrew P. Shacklock;Andrew A. Malcolm;Sim Lay May;Liu Tong;Andrew R. Kennedy.Development of characterisation methodologies for macroporous materials[J].Journal of porous materials,20063/4(3/4):431-438. |
| [6] | 陈宗宗;张瑞丰.基于Polymer-S-C/SiO2多层结构大孔电极锂硫离子电池的制备与性能[J].复合材料学报,2014(2):525-531. |
| [7] | Ratna Balgis;Sumihito Sago;Gopinathan M. Anilkumar.Self-Organized Macroporous Carbon Structure Derived from Phenolic Resin via Spray Pyrolysis for High-Performance Electrocatalyst[J].ACS applied materials & interfaces,201322(22):11944-11950. |
| [8] | Lili Yang;Dengteng Ge;Yanbo Ding;Jiupeng Zhao.Studies on late formation of 3D ordered macroporous materials through colloidal crystal templates[J].Journal of porous materials,20126(6):1023-1026. |
| [9] | Synthesis and characterization of porous glycidylmethacrylate-divinylbenzene monoliths using the high internal phase emulsion approach[J].Reactive & functional polymers,20099(9):724. |
| [10] | Sandi?, Z.P.;Nastasovi?, A.B.;Jovi?-Jovi?i?, N.P.;Milutinovi?-Nikoli?, A.D.;Jovanovi?, D.M..Sorption of textile dye from aqueous solution by macroporous amino-functionalized copolymer[J].Journal of Applied Polymer Science,20111(1):234-242. |
| [11] | Rick C.Schroden;Christopher F.Blandord;Brian J.Melde;Bret J.S.Johnson;Andreas Stein.Direct synthesis of ordered macroporous silica materials functionalized with polyoxometalate clusters[J].Chemistry of Materials,20013(3):1074-1081. |
| [12] | Petra B. Welzel;Milauscha Grimmer;Claudia Renneberg.Macroporous StarPEG-Heparin Cryogels[J].Biomacromolecules,20128(8):2349-2358. |
| [13] | Ouyang H;DeLouise LA;Miller BL;Fauchet PM.Label-free quantitative detection of protein using macroporous silicon photonic bandgap biosensors[J].Analytical chemistry,20074(4):1502-1506. |
| [14] | Tsujino I;Ako J;Honda Y;Fitzgerald PJ.Drug delivery via nano-, micro and macroporous coronary stent surfaces.[J].Expert opinion on drug delivery,20073(3):287-295. |
| [15] | Janshoff A.;Steinem C.;Greiner DP.;Lin VSY.;Gurtner C. Motesharei K.;Sailor MJ.;Ghadiri MR.;Dancil KPS..Macroporous p-type silicon Fabry-Perot layers. Fabrication, characterization, and applications in biosensing[J].Journal of the American Chemical Society,199846(46):12108-12116. |
| [16] | Tao Liu;Yun Liu;Xiaofeng Wang.Improving catalytic performance of Burkholderia cepacia lipase immobilized on macroporous resin NKA[J].Journal of molecular catalysis, B. Enzymatic,20111/2(1/2):45-50. |
| [17] | Ruifeng Zhang;Lele Zhang.Preparation of 3-dimentional skeletal polymer via control of reaction-induced phase separation in epoxy/poly(ethylene glycol) blends[J].Polymer bulletin,20086(6):671-677. |
| [18] | Ruifeng Zhang;Nengbing Long;Lele Zhang.Preparation of 3-dimensional SiO_2 structures via a templating method[J].Thin Solid Films: An International Journal on the Science and Technology of Thin and Thick Films,200924(24):6677-6680. |
| [19] | 沈维云 .磁性二氧化硅载体的合成、表征及对酶的固定化[D].华中科技大学,2007. |
| [20] | K.N.Kilcawley;M.G.Wilkinson;P.F.Fox.Determination of key enzyme activities in commercial peptidase and lipase preparations from microbial or animal sources[J].Enzyme and Microbial Technology,20023(3):310-320. |
| [21] | 龙能兵;王秋景;张瑞丰.大尺寸大孔径C/SiO2复合导电材料的制备[J].复合材料学报,2011(5):119-125. |
| [22] | Qin Jiang;Zhongli Lei.Synthesis and Properties of Immobilized Pectinase onto the Macroporous Polyacrylamide Microspheres[J].Journal of Agricultural and Food Chemistry,20116(6):2592-2599. |
| [23] | 黄增芳;瞿晓岳;陈正.SiO2-PMMA-GMA亚微米复合粒子的制备及其对环氧树脂的改性[J].复合材料学报,2014(5):1148-1153. |
| [24] | Yusdy;Sohan R. Patel;Miranda G.S. Yap.Immobilization of L-lactate dehydrogenase on magnetic nanoclusters for chiral synthesis of pharmaceutical compounds[J].Biochemical Engineering Journal,20091(1):13-21. |
| [25] | Karel Hernandez;Roberto Fernandez-Lafuente.Control of protein immobilization: Coupling immobilization and site-directed mutagenesis to improve biocatalyst or biosensor performance[J].Enzyme and Microbial Technology,20112(2):107-122. |
| [26] | 曲伟光;魏荣卿;何冰芳;刘晓宁;仲玉;陈新营.亲水梳状环氧聚合物载体柔性固定化脂肪酶[J].催化学报,2011(12):1869-1874. |
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