采用膜技术对蛋白质进行分离纯化已受到生命科学领域的广泛关注,而智能开关膜为蛋白质精细分离提供了新的解决方案.本文以接枝甲基丙烯酸-N,N-二甲氨基乙酯(DMAEMA)的乙烯-乙烯醇共聚物(EVAL)分离膜为智能开关膜,对其开关特性与蛋白分离性能进行了研究.结果表明,EVAL接枝膜渗透通量具有显著的开关性,且pH交替重复6个单元以后开关性能保持稳定.在模拟蛋白分子牛血清白蛋白(BSA)与溶菌酶(LZM)及混合模拟蛋白分子BSA与LZM分离过程中,接枝率为15％的EVAL接枝膜在pH3.6的条件下对BSA与LZM分离效果最佳,分离系数分别为9.7和7.8.
参考文献
| [1] | Yao CH.;Chuang WY.;Chen YS.;Young TH..In vitro study of the effect of doxorubicin released from EVAL membrane on vesical cancer cells[J].Materials Chemistry and Physics,20021(1):1-5. |
| [2] | 叶卉 .乙烯-乙烯醇共聚物分离膜制备及其乳清资源化利用研究[D].天津工业大学,2012. |
| [3] | Hong, KH;Liu, N;Sun, G.UV-induced graft polymerization of acrylamide on cellulose by using immobilized benzophenone as a photo-initiator[J].European Polymer Journal,20098(8):2443-2449. |
| [4] | 白丽燕;陈春琴;林东强;姚善泾.蛋白质水力学半径的测定及用于蛋白质变性过程的实时监控[J].化工学报,2008(6):1485-1489. |
| [5] | Changsheng Zhao;Shengqiang Nie;Min Tang.Polymeric pH-sensitive membranes—A review[J].Progress in Polymer Science,201111(11):1499-1520. |
| [6] | High tensibility and pH-responsive swelling of nanocomposite hydrogels containing the positively chargeable 2-(dimethylamino)ethyl methacrylate monomer[J].Reactive & functional polymers,20105(5):p.267. |
| [7] | 杜军;何霞;唐金晶;郑习霞.pH值敏感响应膜的制备和性能研究[J].材料导报,2007(z1):369-372. |
| [8] | Xu FJ;Kang ET;Neoh KG.pH- and temperature-responsive hydrogels from crosslinked triblock copolymers prepared via consecutive atom transfer radical polymerizations[J].Biomaterials,200614(14):2787-2797. |
| [9] | Mahendra Kumar;Mathias Ulbricht.Novel ultrafiltration membranes with adjustable charge density based on sulfonated poly(arylene ether sulfone) block copolymers and their tunable protein separation performance[J].Polymer: The International Journal for the Science and Technology of Polymers,20141(1):354-365. |
上一张
下一张
上一张
下一张
计量
- 下载量()
- 访问量()
文章评分
- 您的评分：
-
10%
-
20%
-
30%
-
40%
-
50%