建立了应用毛细管胶束电动色谱（MECC）灵敏、快速的测定血管紧张素转化酶（ACE）活性的方法。通过对电压、上样时间、电极缓冲液体系等影响因素的优化，探讨了方法的可行性，确立了最佳测定条件(电压：8.1kV；上样时间：1s；电极缓冲液：20mmol/L硼酸盐缓冲液(pH9.0，含50mmol/LSDS)；检测波长：228nm)。方法的最低ACE活性检测限为5pmol/min(以2倍的信噪比计)。
A sensitive and rapid method was developed for angiotensin-converting enzyme (ACE) activity determination by micellar electrokinetic capillary chromat ography (MECC). MECC was carried out to separate and quantify the products of t h e enzymatic reaction using Hip-Leu-His as the substrate in 20 mmol/L boric aci d- borate buffer (pH 9.0) including 50 mmol/L SDS as the run buffer at an applied vo ltage of 8.1 kV. The electrophoresis wa s m onitored at 228 nm, and completed in 6 minutes. The detection limits of ACE act ivity was 5 pmol/min(signal to noise ratio was 2).
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