通过高温干烤和焦碳酸二乙酯(DEPC)两种技术除去核糖核酸(RNA)酶,在保证RNA的稳定性的情况下,在1.0%T,0%C的线性聚丙烯酰胺(即丙烯酰胺和双丙烯酰胺的总质量分数是1.0%,交联度为0%)筛分介质中,利用无胶筛分毛细管电泳技术对水稻中的总RNA进行了分析,整个分析过程在15 min内完成。利用5.0%T,0%C线性聚丙烯酰胺筛分介质,对水稻中的转移RNA(tRNA)进行了较为详细的分析,在30 min内可将tRNA分为两组共9个峰。该方法可用于植物中RNA的分析,具有快速、准确的特点。
: When the RNase was eliminated through baking at 200 ℃ or treated by diethylpyrocarbonate(DEPC), the total RNA of rice could be separated within 15 min using 1.0%T, 0%C linear polyacrylamide as sieving matrix and 7 mol/L urea as denaturant. The tRNA of rice can be separated into two classes and about nine peaks when high concentrated polyacrylamide sieving matrix (5.0%T, 0%C) was used. This technique could provide one of the rapid and accurate methods for the determination of RNA in plants.
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