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通过高温干烤和焦碳酸二乙酯(DEPC)两种技术除去核糖核酸(RNA)酶,在保证RNA的稳定性的情况下,在1.0%T,0%C的线性聚丙烯酰胺(即丙烯酰胺和双丙烯酰胺的总质量分数是1.0%,交联度为0%)筛分介质中,利用无胶筛分毛细管电泳技术对水稻中的总RNA进行了分析,整个分析过程在15 min内完成。利用5.0%T,0%C线性聚丙烯酰胺筛分介质,对水稻中的转移RNA(tRNA)进行了较为详细的分析,在30 min内可将tRNA分为两组共9个峰。该方法可用于植物中RNA的分析,具有快速、准确的特点。

: When the RNase was eliminated through baking at 200 ℃ or treated by diethylpyrocarbonate(DEPC), the total RNA of rice could be separated within 15 min using 1.0%T, 0%C linear polyacrylamide as sieving matrix and 7 mol/L urea as denaturant. The tRNA of rice can be separated into two classes and about nine peaks when high concentrated polyacrylamide sieving matrix (5.0%T, 0%C) was used. This technique could provide one of the rapid and accurate methods for the determination of RNA in plants.

参考文献

[1] Salas-Solano O,Carrilho E,Kotler L,et al. Anal Chem,1998,70:3 996-4 003
[2] Bocek P,Chrambach A.Electrophoresis,1992,13:31-34
[3] Katsivela E,Hfle M G.J Chromatogr A,1995,700:125-136
[4] Skeidsvoll J, Ueland P M. Electrophoresis, 1996, 17: \{1 512\}-1 517
[5] Hjerten S. J Chromatogr, 1985, 347:191-194
[6] Sambrook J, Fritsch E F, Maniatis T. YAN Zi-ying,WANG Hai-lin,translation. Current protocola in molecular biology. Beijing: Science Press, 1998. 126-127Sambrook J, Fritsch E F, Maniatis T. 颜子颖,王海林译. 精编分子生物学实验指南. 北京:科学出版社,1998. 126-127
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