利用高效液相色谱法和荧光检测器建立了水杨酸及其主要的羟基化产物2,3-二羟基苯甲酸和2,5-二羟基苯甲酸的测定方法，并对水杨酸和H\-2O\-2光解反应体系进行了研究。
High performance liquid chromatography (HPLC) with fluorescence detector was used to determine salicylic acid (SA) and its main hydroxylated products, 2,3-dihydroxybenzoic acid (2,3-DHBA) and 2,5-dihydroxybenzoic acid (2,5-DHBA)． Mobile phase was 30 mmol/L acetate(pH 4．9) and the flow rate was 1．0 mL/min． Low detection limits, 10 nmol/L for 2,3-DHBA, 0．6 nmol/L for 2,5-DHBA and 1．0 nmol/L for SA were obtained． The photolysis system of salicylic acid and H\-2O\-2 was investigated． The products in the system were measured and the yields were 45% and 51% for 2,3-DHBA and 2,5-DHBA, respectively．
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