制备反相高效液相色谱法用于从杉材中分离制备倍半萜类化合物

色谱 , 2005, 23(1): 85-87. doi: 10.3321/j.issn:1000-8713.2005.01.019

制备反相高效液相色谱法用于从杉材中分离制备倍半萜类化合物

陈晓辉 ^1, , 张晖芬 ^2, , 毕开顺 ^3, , 金哲史 ^4, , 崛池道郎 ^5,

1.沈阳药科大学药学院,辽宁,沈阳,110016

2.沈阳药科大学药学院,辽宁,沈阳,110016

3.沈阳药科大学药学院,辽宁,沈阳,110016

4.日本爱媛大学生物资源利用学科,日本,高知,783-8502

5.日本爱媛大学生物资源利用学科,日本,高知,783-8502

关键词：制备液相色谱 , 倍半萜类化学成分 , 杉材

Isolation and Preparation of Sesquiterpenols from the Japanese Cedar,Cryptomeria japonica D. Don by Preparative Reversed-Phase High Performance Liquid Chromatography and Its Characterization

利用制备高效液相色谱法从日本产杉材(Cryptomeria japonica D.Don)中分离制备倍半萜类化合物.在YWC-Pack C18柱上,以异丙醇-甲醇-正己烷-水(体积比为50:35:10:5)为流动相,流速为5 mL/min,制备了2种倍半萜类化合物,经紫外光谱、红外光谱及核磁、质谱分析,确认它们分别为(-)-cubebol和(+)-2,7(14),10-bisabolatrien-1-ol-4-one.所制备的两个化合物的纯度分别为98.7%和99.1%.

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制备反相高效液相色谱法用于从杉材中分离制备倍半萜类化合物

Isolation and Preparation of Sesquiterpenols from the Japanese Cedar,Cryptomeria japonica D. Don by Preparative Reversed-Phase High Performance Liquid Chromatography and Its Characterization

参考文献