建立了反相高效液相色谱分离、质谱定性、紫外检测定量测定康肾颗粒中葛根素含量的方法.采用YMC-ODS色谱柱(4.6 mm i.d.×100 mm,10 μm),选用甲醇(含0.1%三氟醋酸)和水(含0.1%三氟醋酸)体系为流动相,非线性梯度洗脱分离,流速1.0 mL/min,检测波长250 nm;用基质辅助激光解吸离子化飞行时间质谱(MALDI-TOF MS)分析鉴定对照品和样品的馏分,在确定目标峰后进行紫外检测定量测定.结果表明,葛根素在0.132 ～1.32 μg范围内其峰面积与进样量呈良好的线性关系(r=0.999 7),平均加标回收率高于103%,相对标准偏差低于2.0%(n=6).应用该方法测定康肾颗粒中葛根素,其平均含量为3.09 mg/g.该方法简单、快速、重现性好.
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