蛋白折叠液相色谱法(PFLC)用于变性蛋白质复性并同时纯化时对流动相组成及其洗脱条件的要求远较通常的液相色谱法高.用端基为PEG-200的高效疏水作用色谱固定相对重组人干扰素-γ(rhIFN-γ)进行纯化并同时复性,详细研究了流动相组成、梯度洗脱模式和流速对rhIFN-γ质量回收率和活性的影响.分别以3.0 mol/L(NH4)2SO4+0.05 mol/L KH2PO4(pH 7.0)和0.05 moL/L KH2PO4(pH 7.0)为流动相A和B,采用35 min非线性梯度洗脱时,所得rhlFN-γ的质量回收率最高.
参考文献
| [1] | Arakawa T,Tsumoto K.Biochem Biophys Res Commun,2003,304(1):148 |
| [2] | Ambrus A,Fesus L.Prep Biochem Biotechnol,2001,31(1):59 |
| [3] | Sparrer H,Rutkat K,Buchner J.Proc Natl Acad Sci USA,1997,94(4):1 096 |
| [4] | Nomura Y,Ikeda M,Yamaguchi N,Aoyama Y,Akiyoshi K.FEBS Letters,2003,553(3):271 |
| [5] | Hagen A J,Hatton T A,Wang D I C.Biotechnol Bioeng,1990,35(10):955 |
| [6] | Roy I,Sharma A,Gupta M N.Biochimica et Biophysica Acta:Proteins and Proteomics,2004,1 698:107 |
| [7] | Geng X D,Bai Q,Wang C Z.Protein Folding Liquid Chromatography.Beijing:Science Press(耿信笃,白泉,王超展.蛋白折叠液相色谱法.北京:科学出版社),2006 |
| [8] | Geng X D,Zhang J,Wei Y M.Chinese Science Bulletin(耿信笃,张静,卫引茂.科学通报),1999,44(19):2 046 |
| [9] | Geng X D,Chang X Q.J Chromatogr,1992,599:185 |
| [10] | Wang L L,Wang C Z,Geng X D.Biotechnol Lett,2006,28(7):993 |
| [11] | Geng X D,Bai Q,Zhang Y J,Li X,Wu D.J Biotechnol,2004,113:137 |
| [12] | Zheng P H,Chen G F,Wang Z W,Pan X G,Lu D R.High Technology Letters(郑平华,陈国富,王正旺,潘学工,陆德如.高技术通讯),1993,10:23 |
| [13] | Guan Y X,Fei Z Z,Luo M,Yao S J Prog Biochem Biophys(关怡新,费峥峥,罗曼,姚善泾.生物化学与生物物理进展),2004,31(10):907 |
| [14] | Shen Y H.[PhD Thesis].Xi'an:Northwest University(申烨华.[博士学位论文].西安:西北大学),2001 |
| [15] | Rubistein P,Familletli C,Sidney P K J.Methods Enzymol,1981,37:755 |
| [16] | Bradford M M Anal Biochem,1976,72:248 |
| [17] | Laemmli U K.Nature,1970,277:680 |
| [18] | Zhang Y J,Li X,Geng X D.Chinese Journal of Chromatography(张养军,李翔,耿信笃.色谱),2001,19(5):423 |
| [19] | Wang Y,Geng X D.Journal of North West University:Natural Science Edition(王彦,耿信笃.西北大学学报:自然科学版),2004,34(1):47 |
| [20] | Geng X D,Bai Q.Science in China:Series B(耿信笃,白泉.中国科学:B辑),2002,32(5):460 |
| [21] | Geng X D,Guo L A,Chang J H.J Chromatogr,1990,507:1 |
| [22] | Chinese Standardized Commission of Biologics.Requirements for Biologics of China Beijing:Chemical Industry Press(中国生物制品标准化委员会.中国生物制品规程.北京:化学工业出版社),2000:384 |
| [23] | Li W,Ou Y F.Biotech Inform,2000(4):37 |
| [24] | Yao W B,Wu D,Geng X D.Chinese Journal of Chromatography(姚文兵,吴丹,耿信笃.色谱),2004,22(2):121 |
上一张
下一张
上一张
下一张
计量
- 下载量()
- 访问量()
文章评分
- 您的评分：
-
10%
-
20%
-
30%
-
40%
-
50%