建立了一种用Ni2+螯合的Chelating Sepharose Fast Flow亲和柱色谱和Sephadex G-75凝胶排阻柱色谱分离纯化重组肿瘤血管生长抑制因子Kringle 5的方法.采用该工艺得到的重组Kringle 5经十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分析表明其纯度约为98%,且具有抑制鸡胚绒毛尿囊膜新生血管生成的生物活性.
参考文献
| [1] | Folkman J,D'Amore P A.Cell,1996,87:1 153 |
| [2] | Davidson D J,Haskell C,Majest S,Kherzai A,Egan D A,Walter K A,Schneider A,Gubbins E F,Solomon L,Chen Z B,Lesniewski R,Henkin J.Cancer Res,2005,65 (11):4 663 |
| [3] | Perri S R,Nalbantoglu J,Annabi B,Koty Z,Beliveau R,Galipeau J.Mol Ther,2004,9:372 |
| [4] | Cai W,Ma J F,Li C Y,Yang Z H,Yang X,Liu W,Liu Z G,Li M T,Gao G Q.J Cell Biochem,2005,96(6):1254 |
| [5] | O'Reilly M S,Holmgren L,Shing Y,Chen C,Rosenthal R A,Moses M,Lane W S,Cao Y H,Sage E H,Folkman J.Cell,1994,79(2):315 |
| [6] | Cao Y H,Ji R W,Davidson D,Schaller J,Marti D,Sohndel S,McCance S G,O'Reilly M S,Llinas M,Folkman J.J Biol Chem,1996,271 (46):29 461 |
| [7] | Cao Y H,Chen A,An S S A,Ji R W,Davidson D,Cao Y M,Llinas M.J Biol Chem,1997,272(36):22 924 |
| [8] | Zhou Y F,Zheng Q,Gao J,Gu J.Biotechnol Lett,2005,27(3):167 |
| [9] | Zhang H X,Fang L,Cheng J,Wei Z,Hua Z C.Prep Biochem Biotechnol,2005,35 (1):17 |
| [10] | Sambrook J,Russell D W.Molecular Cloning.3rd ed.New York:CSHL Press,2001 |
| [11] | Nguyen M,Shing Y,Folkman J.Microvasc Res,1994,47(1):31 |
| [12] | Ribatti D,Vacca A,Roncali L,Dammacco F.Int J Dev Biol,1996,40(6):1 189 |
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