高效液相色谱-串联质谱法分离鉴定绿原酸及其相关杂质

色谱 , 2007, 25(4): 496-500. doi: 10.3321/j.issn:1000-8713.2007.04.010

高效液相色谱-串联质谱法分离鉴定绿原酸及其相关杂质

田晨煦 ^1, , 徐小平 ^2, , 廖丽云 ^3, , 张洁 ^4, , 刘静 ^5, , 周莎 ^6,

1.四川大学华西药学院,四川,成都,610041

2.四川大学华西药学院,四川,成都,610041

3.成都医学院,四川,成都,610083

4.四川大学华西药学院,四川,成都,610041

5.四川大学华西药学院,四川,成都,610041

6.四川大学华西药学院,四川,成都,610041

基金项目: 四川省技术创新项目(成财建[2007]53号)

关键词：高效液相色谱-串联质谱法 , 绿原酸 , 相关杂质 , 鉴别

Separation and Identification of Chlorogenic Acid and Related Impurities by High Performance Liquid Chromatography-Tandem Mass Spectrometry

建立了高效液相色谱-串联质谱法(HPLC-MS/MS)分离和鉴定绿原酸及其相关杂质的方法.采用C18色谱柱(5 μm,4.6 mm×150 mm),乙腈-水(含0.1%甲酸)(体积比为8:92)为流动相,经HPLC-MS/MS和HPLC-二极管阵列检测器在线检测,对工业绿原酸中的奎尼酸、咖啡酸、绿原酸同分异构体等8个相关杂质的结构进行了鉴定.

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材料期刊网

高效液相色谱-串联质谱法分离鉴定绿原酸及其相关杂质

Separation and Identification of Chlorogenic Acid and Related Impurities by High Performance Liquid Chromatography-Tandem Mass Spectrometry

参考文献