亲水作用色谱固定相及其在中药分离中的应用

色谱 , 2009, 27(5): 675-681. doi: 10.3321/j.issn:1000-8713.2009.05.020

亲水作用色谱固定相及其在中药分离中的应用

郭志谋 ^1, , 张秀莉 ^2, , 徐青 ^3, , 梁鑫淼 ^4,

1.中国科学院大连化学物理研究所,中国科学院分离分析重点实验室,辽宁,大连,116023

2.中国科学院大连化学物理研究所,中国科学院分离分析重点实验室,辽宁,大连,116023

3.中国科学院大连化学物理研究所,中国科学院分离分析重点实验室,辽宁,大连,116023

4.中国科学院大连化学物理研究所,中国科学院分离分析重点实验室,辽宁,大连,116023

基金项目: 国家自然科学基金(20775079;20805046)

关键词：亲水作用色谱 , 固定相 , 强极性化合物 , 色谱保留特性 , 中药分离

Stationary phases for hydrophilic interaction liquid chromatography and their applications in separation of traditional Chinese medicines

亲水作用色谱(HILIC)作为一种分离极性化合物的液相色谱模式,近年来越来越受到关注和重视.一方面是因为强极性化合物的分离问题引起了各个研究领域的重视,如药物分析、代谢组学、蛋白质组学等研究领域都不同程度地涉及强极性化合物的分离问题;另一方面是由于HILIC具有流动相组成简单、分离效率较高、与质谱兼容以及反压较低等优势.固定相是HILIC发展和应用的基础,本文主要从固定相分子结构的角度对HILIC固定相的结构特征、保留特性以及应用概况等进行了综述.对传统正相色谱固定相用于HILIC以及专门设计的HILIC固定相进行了介绍,评述了各自的优缺点和应用概况;对近年来HILIC固定相在中药分离中的应用进行了介绍;并对HILIC 固定相的发展进行了展望.

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