通过原位水热合成和溶胶-凝胶浸提涂敷法在碱处理的钛表面制备了HA/CS复合涂层. 接触角检测表明碱处理使钛表面具有超亲水性.X射线衍射分析表明复合涂层成分为HA和CS, 各组分含量由热重分析确定. 用扫描电镜对复合涂层的形貌进行观察,发现不同HA含量的复合涂层具有不同的形貌. 通过培养成骨细胞考察了复合涂层的细胞相容性.Alamar Blue检测表明HA/CS复合涂层表面细胞粘附及增殖能力较好. ALP检测表明HA/CS复合涂层表面的细胞分化能力较好. 综合研究结果表明, 复合涂层有较好的细胞相容性.
Hydroxyapatite/chitosan (HA/CS) composite coatings were prepared on titanium surfaces by hydro-thermal synthesis and Sol-Gel method. The composition, structure, morphology and biocompatibility of composite coatings were characterized. Ti surfaces are convertedinto super-hydrophilic surfaces by alkali treatment, XRD analysis reveals that the coatings are mainly composed of HA and CS and their compositions are determined by TG analysis. SEM observation shows that the coatings with different HA content have different morphologies. Osteoblasts are cultured on the coatings to evaluate their biocompatibility. Alamar Blue assay indicates that cells on the composite coatings has larger proliferation rate than those on pure titanium. Also the ALP activity of the cells on the composite coatings is higher than that on the pure titanium samples. The results demonstrate that as-prepared HA/CS composite coatings have good biocompatibility.
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