通过在湿法合成的二水磷酸氢钙膏体中加入中药骨碎补的提取物,作为磷酸钙骨水泥(Calcium Phosphate Cement,CPC)原料之一,分别制备0、5wt%、10wt%和15wt%的载骨碎补磷酸钙骨水泥.采用Gilmore针、X射线衍射仪、红外光谱仪、万能材料试验机、扫描电子显微镜和紫外分光光度计研究载骨碎补CPC的理化性能和药物释放;体外培养MC-3T3成骨细胞,进行Alamar Blue和碱性磷酸酶检测,研究载骨碎补CPC对成骨细胞增殖和分化的影响,扫描电子显微镜观察细胞形貌.结果表明:随骨碎补浓度的增加,CPC凝结时间明显延长,其抗压强度显著提高;骨碎补促进初期CPC的水化,却阻碍了α-磷酸三钙的转化,且随骨碎补浓度增大作用愈明显,骨碎补不影响CPC水化后的相成分;含骨碎补CPC的微观形貌中出现片状和针状晶体,结构较空白CPC更加致密;药物释放分为突释和缓释两个阶段,符合Higuchi基质扩散释放模型;载骨碎补CPC对成骨细胞的作用呈剂量和时间依赖关系,培养5d时浓度为5wt%和10wt%的CPC较明显地促进细胞增殖,7d时载骨碎补CPC的细胞增殖较稳定,细胞分化能力无显著性差异;成骨细胞在载骨碎补CPC表面生长形态良好,表明该材料具有较好的生物相容性.
参考文献
| [1] | Li J,Hashida Toshiyuki.Preparation of hydroxyapatite ceramics by hydrothermal hot-pressing method at 300℃.J.Mater.Sci.,2007,42(13):5013-5019. |
| [2] | Xu H H K,Carey L E,Simon Jr C G,et al.Premixed calcium phosphate cements:synthesis,physical properties,and cell cytotoxicity.Dental Materials,2007,23(4):433-441. |
| [3] | Vlad M D,Sindilar E V,Mari(n)oso M L,et al.Osteogenic biphasic calcium sulphate dihydrate/iron-modified α-tricalcium phosphate bone cement for spinal applications:in vivo study.Acta Biomaterialia,2010,6(2):607-616. |
| [4] | 李茂红,屈树新,姚宁,等.含丹参磷酸钙骨水泥的制备和表征.生物医学工程与临床,2005,9(3):132-135. |
| [5] | Ginebra M P,Traykova T,Planell J A.Calcium phosphate cements:competitive drug carriers for the musculoskeletal system? Biomaterials,2006,27(10):2171-2177. |
| [6] | Okada S,Ito H,Nagai A,et al.Adhesion of osteoblast-like cells on nanostructured hydroxyapatite.Acta Biomaterialia,2010,6(2):591-597. |
| [7] | 常德有,杨靖,董福慧.四种中药对体外培养大鼠成骨细胞增殖分化功能的影响.辽宁中医杂志,2009,36(8):1420-1422. |
| [8] | 范婧蓉,韩博,李禹涛.骨碎补水提液对大鼠成骨细胞的影响.中国兽医杂志,2009,45(2):13-15. |
| [9] | 高颖,王新峦,王乃利,等.骨碎补中的化学成分.中国药物化学杂志,2008,18(4):284-287. |
| [10] | Jeong J C,Lee J W,Yoon C H,et al.Stimulative effects of drynariae rhizoma extracts on the proliferation and differentiation of osteoblastic MC3T3-E1 cells.J.Ethnopharmacology,2005,96(3):489-495. |
| [11] | Xu YZ,Gao Y B,Guo J J,etal.Effects of different concentrations of thizoma drynariae decoction on proliferation and differentiation as well as ultrastructural change of human dental pulp cells cultured in vitro.J.Clin.Rehabit Tissue Eng.Res.,2009,13(2):324-328. |
| [12] | Sun J S,Dong G C,Lin C Y,et al.The effect of Gu-SUi-Bu (drynaria fortunei J.Sm) on bone cell activities.Biomaterials,2002,23(16):3377-3385. |
| [13] | 姜红江,黄相杰,谭远超,等.磷酸钙骨水泥复方丹参缓释体的制备及性能评价.中国修复重建外科杂志,2007,21(10):1113-1117. |
| [14] | 邹琴,张利,左奕,等.载黄芪多糖骨水泥的理化性能及体外细胞相容性研究.功能材料,2008,39(9):1515-1521. |
| [15] | 苗波,隋长德,李慕勤,等.羟基磷灰石/骨碎补复合材料修复骨缺损的实验研究.黑龙江医药科学,2009,32(2):3-4. |
| [16] | Oreffo R O C,Driessens F C M,Planell J A,et al.Growth and differentiation of human bone marrow osteoprogenitors on novel calcium phosphate cements.Biomaterials,1998,19(20):1845-1854. |
| [17] | Qu S X,Weng J,Feng B,et al.Preliminary study of calcium phosphate immobilized with Chinese medicine,J.Mater.Sci.2005,40(11):3035-3037. |
| [18] | 李茂红,屈树新,姚宁,等(LI Mao-Hong,et al).载不同浓度香丹注射液磷酸钙骨水泥性能研究.无机材料学报(Journal of Inorganic Materials),2010,25(5):507-511. |
| [19] | Khairoun I,Driessens F C M,Boltong M G,et al.Addition of cohesion promotors to calcium phosphate cements.Biomaterials,1999,20(4):393-398. |
| [20] | Chang E J,Lee W J,Cho S H,et al.Proliferative effects of flavan-3-ols and propelargonidins from rhizomes of drynaria fortunei on MCF-7 and osteoblastic cells.Arch Pharm Res.2003,26(8):620-630. |
| [21] | 黄粤,刘昌胜,邵慧芳,等.萘普生钠/磷酸钙骨水泥药物缓释体系的研究.药学学报,2005,35(1):44-47. |
| [22] | Khairoun I.Boltong M G,Driessens F C M,et al.Effect of calcium carbonate on the compliance of an apatitic calcium phosphate bone cement.Biomaterials,1997,18(23):1535-1539. |
| [23] | 沈卫,刘昌胜,顾燕芳,等(SHEN Wet,et al).磷酸钙骨水泥的水化反应机理研究.无机材科学报(Journal of Inorganic Materials),1996,11(4):685-690. |
| [24] | 郭悦华,屈树新,李茂红,等(GUO Yue-Hua,et al).载双组分药物磷酸钙骨水泥的性质和体外释放行为.硅酸盐学报(Journal of the Chinese Ceramic Society),2010,38(3):472-477. |
| [25] | 刘昌胜,刘子胜,潘颂华(LIU Chang-Sheng,et al).磷酸钙骨水泥的水化放热行为.硅酸盐学报(Journal ofthe Chinese Ceramic Society,2000),28(6):501-506. |
| [26] | 郜成莹,叶建东.添加剂和载药方法对庆大霉素/磷酸钙骨水泥载药体系性能及药物体外释放的影响.硅酸盐通报,2008,27(2):225-229. |
| [27] | Deepak K P,Rajalaxmi D,Prasad R C,et al.Synthesis and sintered properties evaluation of calcium phosphate ceramics.J.Mater.Sci.Eng.C,2007,27(4):684-690. |
| [28] | 黄粤,刘昌胜.具有药物缓释功能的磷酸钙骨水泥的研究进展.硅酸盐通报,1999(2):42-47. |
| [29] | Ginebra M P,Traykova T,Planell J A.Calcium phosphate cements as bone drag delivery system:a review.J.Control Release,2006,113(2):102-110. |
| [30] | 杨阳,刘宝瑞,钱晓萍.ALamar Blue用于体外培养细胞活性检测的方法研究.现代肿瘤医学,2006,14(1):6-8. |
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