掺锶羟基磷灰石的骨缺损修复效果优于羟基磷灰石. 以四水硝酸钙[Ca(NO3)2·4H2O]、磷酸氢二铵[(NH4)2HPO4]、硝酸锶[Sr(NO3)2]为原料, 180℃下水热处理8h制得Sr-HA. 通过XRD、FTIR、TEM、EDX和TG/DTA对Sr-HA的晶相、化学组成、形貌和热稳定性进行分析, 并通过细胞培养, MTT比色法对材料的细胞毒性进行评价.结果表明: Sr取代部分Ca进入HA的晶格中. 随着Sr含量的增加, Sr-HA晶粒尺寸呈减小趋势, 热稳定性下降. 掺锶对羟基磷灰石细胞毒性影响较小, 锶磷灰石具有良好的生物相容性.
The biological HA in the body differs from that of pure HA in stoichiometry,composition, physical properties and mechanical properties, because this HA isparticularly prone to ion substitution. As a cation that can substitute forcalcium in the structure of hydroxyapatite, strontium provokes an increasinginterest because of its beneficial effect on bone formation, and prevention ofbone resorption. Here, strontium-substituted HA powders were prepared by thehydrothermal method at 180℃ for 8 h using Ca(NO3)2·4H2O, (NH4)2HPO4and Sr(NO3)2 as reagents. X-ray diffraction, Fouriertransform infrared spectroscope, transmission electron microscope, energy dispersiveX-ray, and thermogravimetric-differential thermal analysis were employed toinvestigate the crystalline phase, chemical composition, morphology, and thermalstability of the Sr-HA. And the cytotoxicity of Sr-HA was analyzed through MTT assay. The results showthat Sr is incorporated into the HA crystal structure and the crystal grainsize of the Sr-HA decreases as the Sr content (under 50wt%) is increased.Moreover, the HA thermal stability decreases. However, there’s no apparentdifference between the pure hydroxyapatite and all of the Sr-HA groups in cytotoxicity,which may have good biocompatibility.
参考文献
| [1] | |
| [2] | Shi Donglu. Introduction to Biomaterials. Beijing: Tsinghua UniversityPress. 2006: 16-28.[2] Blake GM, Fogelman I. Long-term effect of strontium ranelate treatmenton BMD. J. Bone Miner. Res., 2005, 20(11): 1901-1904.[3] Hott M, Deloffre P, Tsouderos Y, etal. S12911-2 reduces bone loss induced by short-term immobilization inrats. Bone, 2003, 33(1): 115-123.[4] Compston J. Prevention of vertebral fractures by strontium ranelate inpostmenopausal women with osteoporosis. OsteoporosInt., 2005, 16(suppl 1): 4-6.[5] Kendler D L. Strontium ranelate—data on vertebral and nonvertebral fractureeffi |
- 下载量()
- 访问量()
- 您的评分：
-
10%
-
20%
-
30%
-
40%
-
50%