采用脉冲电化学沉积法制备磷酸钙/明胶(CaP/Gelatin)复合涂层, 对其进行表征及性能测试, 旨在得到综合性能好的生物医用复合涂层. 研究中讨论了不同脉冲电位、明胶浓度、沉积温度、电解液pH值等对复合涂层性能的影响. 研究发现脉冲电位下涂层质量优于恒电位. XRD和FTIR分析表明复合涂层的成分为羟基磷灰石和明胶. SEM观察发现复合涂层是结构均匀的多孔网状或花瓣状微纳结构. 复合涂层形貌及成分随脉冲电位、明胶浓度等实验条件的变换而变化. 低电位利于复合涂层的结晶和沉积. 改变明胶在钙磷电解液中的浓度, 发现明胶浓度在0.1~1.0 g/lL范围内不影响涂层形貌, 但影响明胶在复合涂层中的含量. 沉积温度为50 ℃, 电解液pH值在明胶等电点以下保证了复合涂层具有较好的结晶性、均匀性. Alamar Blue检测表明复合涂层具有较好的生物相容性.
Bioactive calcium phosphate (Ca-P)coated titanium is the most promising bone replacement materials. Bioactive calciumphosphate (Ca-P) coated titanium has the advantages of both metal and ceramicsand has been considered as one of the most promising bone replacementmaterials. Gelatin is the denatured form of collagen and is expected to bebeneficial for hard tissue applications. The CaP/gelatin composite coatings couldcombine the bioactivity and osteoconductivity of CaP with the good characteristicsof gelatin. In the present study, The pulsed electrochemicaldeposition method was employed to deposit CaP/gelatin composite coatings ontitanium substrates. The main purpose of the study is to explore the optimumprocessing parameters of the composite coating. Electrolyte was made from themixture of the Ca-P and gelatin aqueous solution with different concentrations.SEM, XRD and FTIR were usedto characterize the composition and morphology of the coating. The coatings prepared underconstant voltage and pulse voltage electrodeposition were compared and it was foundthat the pulse voltage mode was more suitable for preparing CaP/gelatincoatings. The results indicated that Theoptimized experimental conditions were as follows,:pulse voltage range, of 0 to -1.3V, the Ca2+ concentrationof 5.0×10-4 mol/L; gelatin concentration of,0.5g/L, pH value, of 5.0; temperature of,50℃. Osteoblasts were cultured on the coatings to evaluate the biocompatibilityof coatings. Alamar Blue assay indicated that the composite coatings couldfavor the proliferation of the osteoblasts.
参考文献
| [1] | 王英波, 鲁 雄, 冯 波, 等(WANG Ying-Bo, et al). 脉冲电沉积制备HA/ZrO2纳米复合涂层的稳定性及生物相容性评价[J]. 高等学校化学学报(Chem. J. ChineseU.), 2010, 31(2): 253-259. [2] Tian Ang, Wang Chao, Xue Xiangxin, et al. Inhibitory effect of nano-ha particles on humanu87 glioblastoma cells viability[J].Journal of Inorganic Materials, 2010, 25(1): 101-106.[3] Weiner S, Wagner H D. The material bone: structure-mechanicalfunction relations[J]. Ann. Rev. Mater. Sci., 1998, 28(1): 271-298. [4] Manso M, Jimenez C, Morant C, etal. Electrodeposition of hydroxyapatite coatings in basic conditions[J].Biomaterials, 2000, 21(17): 1755-1761. [5] Redepenning J, Mclssac J P. Electrocrystallization of rushite coatinson prosthetic alloys[J]. Chem. Mater., 1990, 2(6): 625-627. [6] Hu H B, Lin C J, Hu R, et al.A study on hybrid bioceramic coatings of HA/poly (vinyl acetate) co-depositedelectrochemically on Ti-6A1-4V alloy surface[J], Mater. Sci. Eng. C, 2002, 20(1-/2): 209-214. [7] Mohamed K R, Mostafa A A. Preparation andbioactivity evaluation of hydroxyapatite-titania/chitosan-gelatin polymericbiocomposites[J]. Mater. Sci. Eng.C, 2008, 28(7): 1087-1099. [8] Fan Y, Duan K, Wang. R. A compositecoating by electrolysis-induced collagen self-assembly and calcium phosphatemineralization[J]. Biomaterials, 2005, 26(14): 1623-1632. [9] Kuo M C, Yen S K. The process of electrochemical deposited hydroxyapatitecoatings on biomedical titanium at room temperature. Mater. Sci. Eng. C, 2002, 20(1/2): 153-160.[10] 王英波, 鲁 雄, 翁 杰, 等(WANG Ying-Bo,et al). 脉冲电化学沉积制备n-HA/ZrO2复合涂层[J]. 稀有金属材料与工程(Rare Metal Mat. Eng.),2009, 38(6): 1071-1075. [11] 张柏林, 王英波, 鲁 雄, 等(ZHANG Bai-Lin, etal). 脉冲电化学沉积法制备钛基HA/Ag复合涂层[J].稀有金属材料与工程(Rare MetalMat. Eng.), 2010, 39(10): 1835-1839.[12] Ban S, Maruno S. Effect of temperature on electrochemical depositionof calcium phosphate coatings in a simulated body fluid[J]. Biomaterials,1995, 16(13): 977-981.[13] Hou X H, Liu X, Xu J M, et al. Aself-optimizing electrodeposition process for fabrication of calcium phosphatecoatings[J]. Mater. Lett.,2001, 50(2-/3): 103-107.[14]Manara S, Paolucci F, PalazzoB, at et el.Electrochemically-assisted deposition of biomimetic hydroxyapatite–collagencoatings on titanium plate[J]. Inorg. Chim. Acta, 2008, 361(6): 1634-1645. |
- 下载量()
- 访问量()
- 您的评分：
-
10%
-
20%
-
30%
-
40%
-
50%