采用透射电子显微镜(TEM)和纳米粒度仪比较研究了经不同孔径的微孔滤膜过滤后, 草酸钙结石患者和健康对照者尿液中不同尺寸尿微晶的平均粒径()、自相关曲线、聚集状态和稳定性随放置时间的变化. 随着放置时间从0增加到4 h, 经孔径为0.22、0.45、1.2和3 μm的滤膜过滤后的草酸钙结石患者的尿微晶的均随放置时间的延长而快速增大, 放置4 h后的增幅(Δ)分别为1439、1557、1534和1051 nm; 而对照者的Δ增幅较小, 分别为474、511、567和433 nm, 表明患者尿微晶比对照者的团聚速度快, 团聚程度大, 其稳定性比对照者的差. 小尺寸的尿微晶均更容易团聚. 研究结果表明, 尿微晶的快速团聚是草酸钙结石形成的重要原因.
Changes of average size (), autocorrelation curve, aggregation state and stability of urinary crystallites in urine samples of calcium oxalate (CaOxa) stone patients and healthy controls with placement time were comparatively studied using transmission electron microscopy (TEM) and nanoparticle size analyzer. The urine samples were filtrated by microporous membrane with a pore size of 0.22, 0.45, 1.2 and 3 mm, respectively. With the increase of placement time from 0 to 4 h, the values of the crystallites in various filtrated urine of patients increased rapidly. The amplification of (Δ) was 1439, 1557, 1534 and 1051 nm, respectively, when the placement time was up to 4 h. However, the Δof the urine crystallites of the controls was relatively small, which was only 474, 511, 567 and 433 nm, respectively. The urinary crystallites of the patients had rapid and large agglomeration and poor stability compared with those of the controls. The urinary crystallites of small size agglomerated more easily than those of large size. The results show that the rapid agglomeration of urine crystallites may play an important role in the formation of CaOxa stones.
参考文献
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