为在磷灰石-硅灰石生物活性玻璃陶瓷(Apatite-Wollastonite Bioactive Glass-Ceramic,AW)表面引入能够促进细胞粘附的RGD(精氨酸-甘氨酸-天冬氨酸)多肽以提高其生物活性,采用低温等离子法在材料的表面引入活性氨基基团,并通过浸渍法使氨基基团与多肽发生反应.采用XRD、XPS、ATR-FTIR对AW的相组成及表面改性特性进行表征,确认通过低温等离子法在AW表面接上氨基,RGD多肽分子与氨基反应以化学键合的形式接枝到材料表面(RGD-AW),实现了在AW表面接枝生物大分子的改性.将改性前后的材料分别与类成骨细胞(MG63细胞)混合培养并使用荧光显微镜、SEM及MTT等测试方法对材料的细胞生物学性能进行了表征.细胞实验结果表明:接枝RGD多肽分子的材料在细胞培养的早期阶段比AW更有利于细胞的粘附及铺展.
参考文献
| [1] | Wang X,Li X,Ito A,et al.Synthesis and characterization of hierarchically macroporous and mesoporous CaO-MO-SiO2-P2O5 (M=Mg,Zn,Sr) bioactive glass scaffolds.Acta Biomaterialia,2011,7(10):3638-3644. |
| [2] | Bagno A,Piovan A,Dettin M,et al.Human osteoblast-like cell adhesion on titanium substrates covalently functionalized with synthetic peptides.Bone,2007,40(3):693-699. |
| [3] | Meyers S R,Grinstaff M W.Biocompatible and bioactive surface modifications for prolonged in vivo efficacy Chemical Reviews,2012,112(3):1615-1632. |
| [4] | Prestwich GD,Erickson I E,Zarembinski T I,et al.The translational imperative:making cell therapy simple and effective.Acta Biomaterialia,2012,8(12):4200-4207. |
| [5] | Yan C,Sun J,Ding J.Critical areas of cell adhesion on micropatterned surfaces.Biomaterials,2011,32(16):3931-3938. |
| [6] | Hynes R O.Integrins:bidirectional,allosteric signaling machines.Cell,2002,110(6):673-687. |
| [7] | Collier J H,Segura T.Evolving the use of peptides as components of biomaterials.Biomaterials,2011,32(18):4198-4204. |
| [8] | Williams D F.The role of short synthetic adhesion peptides in regenerative medicine; the debate.Biomaterials,2011,32(18):4195-4197. |
| [9] | Ruoslahti E.RGD and other recognition sequences for integrins.Annual Review of Cell and Developmental Biology,1996,12:697-715. |
| [10] | Dreier B,Raghunathan V K,Russell P,et al.Focal adhesion kinase knockdown modulates the response of human corneal epithelial cells to topographic cues.Acta Biomaterialia,2012,8(12):4285-4294. |
| [11] | Hermessy K M,Pollot B E,Clem W C,et al.The effect of collagen I mimetic peptides on mesenchymal stem cell adhesion and differentiation,and on bone formation at hydroxyapatite surfaces.Biomaterials,2009,30(10):1898-1909. |
| [12] | Mark K,Park J,Bauer S,et al.Nanoscale engineering of biomimetic surfaces:cues from the extracellular matrix.Cell Tissue Res.2010,339(1):131-153. |
| [13] | Ceylan H,Tekinay A B,Guler M O.Selective adhesion and growth of vascular endothelial cells on bioactive peptide nanofiber functionalized stainless steel surface.Biomaterials.2011,32(34):8797-8805. |
| [14] | Park J W,Kurashima K,Tustusmi Y,et al.Bone healing of commercial oral implants with RGD immobilization through electrodeposited poly(ethylene glycol) in rabbit cancellous bone.Acta Biomaterialia,2011,7(8):3222-3229. |
| [15] | Su B H,Ran J G,Chen Z Q.Study on increasing bioactivity of Hydroxy-poly-calcium sodium phosphate appatite modified by cold plasma technique.Space Medicine & Medical Engineering,2003,16(1):68-71. |
| [16] | Li G D,Zhou D L,Feng D G,et al.Preparation and properties of magnetic bioactive glass-ceramics doped with Mn-Zn ferrite.Journal of Inorganic Materials,2008,23(3):621-625. |
| [17] | Xue M,Feng D G,Zhou D L,et al.Preparation of porous apatite-wollastonite bioactive glass ceramic (AW-GC) by dipping with polymer foams.Chinese Journal of Inorganic Chemistry,2007,23(4):708-712. |
| [18] | Chen Q Z,Thouas G A.Fabrication and characterization of Sol-Gel derived 45S5 bioglass(R)-ceramic scaffolds.Acta Biomaterialia,2011,7(10):3616-3626. |
| [19] | De Bartolo L,Morelli S,Lopez L C,et al.Biotransformation and liver-specific functions of human hepatocytes in culture on RGD-immobilized plasma-processed membranes.Biomaterials,2005,26(21):4432-4441. |
| [20] | Marelli B,Ghezzi C E,Mohn D,et al.Accelerated mineralization of dense collagen-nano bioactive glass hybrid gels increases scaffold stiffness and regulates osteoblastic function.Biomaterials,2011,32(34):8915-8926. |
| [21] | Goel A,Rajagopal R R,Ferreira J M.Influence of strontium on structure,sintering and biodegradation behaviour of CaO-MgO-SrO-SiO2-P2O5-CaF2 glasses.Acta Biomaterialia,2011,7(11):4071-4080. |
| [22] | Ji J,Bar-On B,Wagner H D.Mechanics of electrospun collagen and hydroxyapatite/collagen nanofibers.Journal of the Mechanical Behavior of Biomedical Materials,2012,13:185-193. |
| [23] | Shiwaku Y,Anada T,Yamazaki H,et al.Structural,morphological and surface characteristics of two types of octacalcium phosphate-derived fluoride-containing apatitic calcium phosphates.Acta Biomaterialia,2012,8(12):4417-4425. |
| [24] | Hall A.Rho GTPases and the actin cytoskeleton.Science,1998,279(23):509-514. |
| [25] | Yang C,Czech L,Gerboth S,et al.Novel roles of formin mDia2 in lamellipodia and filopodia formation in motile cells.PLoS Biology,2007,5(11):e317. |
上一张
下一张
上一张
下一张
计量
- 下载量()
- 访问量()
文章评分
- 您的评分：
-
10%
-
20%
-
30%
-
40%
-
50%