研究了混合溶剂体系中有机溶剂种类和含量对牛肝β-半乳糖苷酶催化5-氟-2'-脱氧尿苷区域选择性半乳糖基化反应的影响.结果表明,在含10%(体积分数)有机溶剂体系中,该酶稳定性差,失活严重.减少有机溶剂含量可显著减轻其对该酶的毒害作用.然而,有机溶剂的添加对酶促糖基化反应的区域选择性的影响很小(均保持在99%以上).牛肝β-半乳糖苷酶在混合溶剂体系中的催化性能与有机溶剂的介电常数有良好的相关性,其中在含二甲亚砜(DMSO)体系中该酶催化活性最高.当体系中DMSO含量≤5%时,目标产物收率达71%.
参考文献
| [1] | Chabner B A;Ryan D P;Paz-Ares L;Garcia-Carbonero R,Calabresi P.Hardman J G,Limbird L E eds.Goodman & Gilman's The Pharmacological Basis of Therapeutics[M].New York:McGraw-Hill,2001:1389. |
| [2] | Watanabe K A;Matsuda A;Halat M J;Hollenberg D H Nisselbaum J S Fox J J .[J].Journal of Medicinal Chemistry,1981,24:893. |
| [3] | Abraham R;Aman N;von Borstel R;Darsley M Kamireddy B Kenten J Morris G Titmas R .[J].Cell Biochemistry and Biophysics,1994,24:127. |
| [4] | Efimtseva E V;Mikhailov S N .[J].Russian Chemical Reviews,2004,73:401. |
| [5] | Efimtseva E V;Kulikova I V;Mikhailov S N .[J].Molecular Biology,2009,43:301. |
| [6] | De Clercq E;Field H J .[J].British Journal of Pharmacology,2006,147:1. |
| [7] | Kim SH;Yoo SM;Park IS;Kim YH .A new inosine disaccharide from the crustacean Ligia exotica: isolation and structure elucidation by total synthesis.[J].Journal of natural products,2000(9):1188-1191. |
| [8] | Efimtseva E V;Kulikova I V;Mikhailov S N .[J].Current Organic Chemistry,2007,11:337. |
| [9] | Liu Z J;Zhou M;Min J M;Zhang L H .[J].TETRAHEDRON-ASYMMETRY,1999,10:2119. |
| [10] | Binder W H;Kahlig H;Schmid W .[J].TETRAHEDRON-ASYMMETRY,1995,6:1703. |
| [11] | Andreotti G;Trincone A;Giordano A .Convenient synthesis of beta-galactosyl nucleosides using the marine beta-galactosidase from Aplysia fasciata[J].Journal of molecular catalysis, B. Enzymatic,2007(1/2):28-32. |
| [12] | Zeng Q M;Li N;Zong M H .[J].Biotechnology Letters,2010,32:1251. |
| [13] | Li N;Smith T J;Zong M H .[J].Biotechnology Advances,2010,28:348. |
| [14] | Shin H J;Yang J W .[J].Biotechnology Letters,1994,16:1157. |
| [15] | Yoon JH.;Rhee JS. .The efficient enzymatic synthesis of N-acetyllactosamine in an organic co-solvent[J].Carbohydrate research,2000(4):377-383. |
| [16] | Hansson T;Adlercreutz P .[J].Biotechnology and Bioengineering,2001,75:656. |
| [17] | Bridiau N;Issaoui N;Maugard T .[J].Biotechnology Progress,2010,26:1278. |
| [18] | Kaftzik N;Wasserscheid P;Kragl U .[J].Organic Process Research & Development,2002,6:553. |
| [19] | Affleck R;Haynes C A;Clark D S .[J].Proceedings of the National Academy of Sciences of the United States of America,1992,89:5167. |
| [20] | Li N;Zong MH;Liu XM;Ma D .Regioselective synthesis of 3 '-O-caproyl-floxuridine catalyzed by Pseudomonas cepacia lipase[J].Journal of molecular catalysis, B. Enzymatic,2007(1/2):6-12. |
| [21] | Dean J A.Lange's Handbook of Chemistry[M].New York:McGraw-Hill,1998:105. |
| [22] | Laane C;Boeren S;Vos K;Veeger C .[J].Biotechnology and Bioengineering,1987,30:81. |
| [23] | Prodanovic R;Milosavic N;Jovanovic S;Prodanovic O Velickovic T C Vujcic Z Jankov R M .[J].Biocatalysis and Biotransformation,2006,24:195. |
| [24] | Majumder AB;Singh B;Gupta MN .Diastereoselective synthesis of (R)-(alkyl)-beta-D-galactopyranoside by using beta-galactosidase (Aspergillus oryzae) in low-water media.[J].Bioorganic and Medicinal Chemistry Letters,2008(1):124-128. |
| [25] | Knapp S;Dong Y;Rupitz K;Withers S G .[J].Bioorganic and Medicinal Chemistry Letters,1995,5:763. |
| [26] | Lang M;Kamrat T;Nidetzky B .Influence of ionic liquid cosolvent on transgalactosylation reactions catalyzed by thermostable beta-glycosylhydrolase celB from Pyrococcus furiosus[J].Biotechnology and Bioengineering,2006(6):1093-1100. |
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