研究了有机溶剂/缓冲液双相体系中绿豆环氧化物水解酶高立体选择性地催化外消旋环氧苯乙烯水解生成(R)-苯基乙二醇反应.结果表明,与单水相反应体系相比,有机溶剂/缓冲液双相反应体系不仅有效地抑制环氧苯乙烯的非酶水解反应,而且明显提高底物的浓度,产物的收率和ee值更高.在所考察的不同有机溶剂中,正己烷不仅能较好地溶解底物,而且对酶的毒性较小,从而导致反应的初速率较快,产物的收率和ee值较高,是最适宜有机相.在两相体积比(正己烷/缓冲液)为1:1,底物浓度为20 mmol/L,缓冲液pH=6.5以及35℃的最适反应条件下,反应的初速率、产物的收率和ee值分别为5.42 mmol/(L·h),49.2％和94.3％.
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