以紫外光表面接枝改性的聚乙烯(PE)中空纤维膜为载体,采用共价结合的方式固定化甲酸脱氢酶(FDH),考察了CO2通入方式、溶液pH值、缓冲液种类和还原型烟酰胺腺嘌呤二核苷酸(NADH)的浓度对酶催化CO2合成甲酸反应的影响.结果表明,与加压法相比,CO2鼓泡法更有利于甲酸的生成；磷酸盐缓冲液优于Tris-HCl和盐酸三乙醇胺缓冲液；体系pH值对反应的影响较大,固定化FDH的最佳pH值仍为6.0,但pH耐受性增强;随着辅酶NADH浓度的增加,反应初速度加快,收率下降；游离酶和固定化酶的最大酶活分别为0.246和0.138 mmol/(L·h);固定化FDH在4C贮存两周后活性仅下降4％,而游离酶活性下降50％.FDH催化膜重复利用10次后,活性没有明显降低.
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