运用毛细管电色谱(pCEC)模式,以羟丙基-β-环糊精(HP-β-CD)作为手性选择剂,对1-甲基-3-苯基丙胺对映体进行手性分离.1-甲基-3-苯基丙胺对映体在最佳的条件下如:手性选择剂浓度10 g/L、流动相配比:V(乙腈):V(磷酸盐体系)=60:40溶液(5 mmol/L)、背景电解质pH=7.6、柱温16 ℃和分离电压10 kV达到了基线分离,该方法重现性好、简便、快捷.
1-Dimethyl-3-phenylpropylamine enantiomer was separated with hydroxypropyl-β-cyclodextrin(HP-β-CD) as chiral selector by means of pressurized capillary electrochromatography(pCEC). The optimal experimental conditions are HP-β-CD concentration 10 g/L, background buffer pH=7.6, capillary temperature 16 ℃, voltage 10 kV, and the mobile phase consisted of a solution of acetonitrile and phosphate at a volume ratio of V(CH_3CN):V(NaH_2PO_4-Na_2HPO_4)=60:40. The method is simple, rapid and reproducible.
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