利用循环伏安法,研究了银和L-天冬氨酸在玻碳电极表面电化学聚合的条件,制备了银掺杂聚L-天冬氨酸修饰电极.研究了肾上腺素在修饰电极上的电化学行为,建立了循环伏安法测定肾上腺素的新方法.在pH=3.5的磷酸盐缓冲溶液中,扫描速率为50 mV/s时,肾上腺素在修饰电极上产生一对明显的氧化还原峰,峰电位分别为E_(pa)=0.447 V,E_(pc)=0.387 V.用循环伏安法测定时,氧化峰电流与肾上腺素浓度分别在8.00×10~(-8)～1.00×10~(-5) mol/L和1.00×10~(-5)～1.00×10~(-4) mol/L范围内呈良好的线性关系,检出限为8.0×10~(-9) mol/L.
A silver doped poly(L-aspartic acid) modified electrode (Ag-PLA/GCE) was prepared by electrochemical immobilization of silver and L-aspartic acid on a glassy carbon electrode surface. The polymerization conditions and the electrochemical behavior of epinephrine were studied. A novel cyclic voltammetric method was proposed for the determination of epinephrine. In a pH=3.5 phosphate buffer solution,a pair of redox peaks were observed at peak potential E_(pa)=0.447 V and E_(pc)=0.387 V(vs.Ag/AgCl) at a scan rate of 50 mV/s. By cyclic voltammetry,the oxidation peak current of epinephrine was proportional to the concentration in a range of 8.00×10~(-8)～1.00×10~(-5) mol/L and that of 1.00×10~(-5)～1.00×10~(-4) mol/L. The detection limit was 8.0×10~(-9) mol/L.
参考文献
| [1] | XUE Qi-Ming(薛启冥).Physiological and Pathological Chemistry of nervous System(神经系统的生理和病理化学)[M].Beijing(北京):Science Press(科学出版社),1978:102 |
| [2] | ZUO Guo-Fang(左国防),YANG Jian-Dong(杨建东),YUAN Hui-Qing(原慧卿),LIU Xiu-Hui(刘秀辉),LU Xiao-Quan(卢小泉).J Instrum Anal(分析测试学报)[J],2006,25(6):46 |
| [3] | LI Hong-Kun(李洪坤),JIA Jing-Jing(贾晶晶),LI Jun(李军),ZHANG Guo-Rong(张国荣).Chinese J Anal Lab(分析试验室)[J],2007,26(1):89 |
| [4] | TANG Ping(唐平),ZENG Bai-Zhao(曾百肇).Chinese J Anal Chem(分析化学)[J],2003,31(6):641 |
| [5] | FEI Jun-Jie(费俊杰),TAN Hong-Yan(谭红艳),CHEN Xiao-Ming(陈小明),LI Ju-Nan(黎拒难).Chinese J Catal(催化化学)[J],2006,27(7):619 |
| [6] | JIN Bao-Kang(金葆康),WANG Shi-Jun(王世君),ZHANG Zi-Pin(张自品).J Anhui Univ(Nat Sci Edn)(安徽大学学报(自然科学版))[J],2005,19(5):71 |
| [7] | Hu W N,Sun D M,Ma W.Chem Anal(Warsaw)[J],2008,53(5):703 |
| [8] | LIN Li(林丽),QIU Pei-Hong(仇佩虹),YANG Li-Zhu(杨丽珠),CAO Xu-Ni(曹旭妮),JIN Li-Tong(金利通).Chinese J Anal Chem(分析化学)[J],2006,34(1):31 |
| [9] | MA Wei(马伟),SUN Deng-Ming(孙登明).Acta Phys-Chim Sin(物理化学学报)[J],2007,23(3):332 |
| [10] | Laviron E.J Electroanal Chem[J],1979,101(1):19 |
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