采用复合酶法提取和二乙基氨基乙基(DEAE)-纤维素52(DE-52)柱层析纯化制得茶多糖复合物TPC-1;采用煎煮提取、Sephadex G-200柱层析纯化获得茶多糖(TPC)复合物TPC-2.高效液相凝胶渗透色谱-蒸发光散射检测表明,TPC-1和TPC-2经98℃水浴处理5 h后,TPC-1的主要均一性组分TPC-1a裂变为TPC-1a-1和TPC-1a-2 2个组分;圆二色(CD)谱显示TPC-1在194 nm呈现1个正Cotton效应峰,热处理后在216 nm处增加1个正Cotton效应峰;TPC-2经热处理后其中2种均一性组分TPC-2a和TPC-2b未变化,CD谱显示TPC-2在203、215和272 nm处均呈现显著的的正Cotton效应峰,经热处理后前2个峰消失.98℃水浴热处理改变了低温复合酶法提制的茶多糖复合物TPC-1溶液构象和均一性组分的分布,未改变沸水煎煮提制的TPC-2的均一性组分的分布和凝胶色谱行为,但影响了其溶液构象.
参考文献
| [1] | DING Renfeng,HE Puming,JIE Guoliang.Study on the Hypoglycemic Mechanism of Tea Polysaccharides and Tea Polyphenols[J].J Tea Sci,2005,25(3):219-224(in Chinese).丁仁凤,何普明,揭国良.茶多糖和茶多酚的降血糖作用研究[J].茶叶科学,2005,25(3):219-224. |
| [2] | WANG Yuanfeng,JIN Zhengyu.Chemical Property and Hypoglycemic Effects of Acidic Tea Polysaccharides[J].Nat Prod Res Dev,2005,17(4):424-427,456(in Chinese).王元凤,金征宇.茶叶中酸性杂多糖的部分化学性质及降血糖活性的研究[J].天然产物研究与开发,2005,17(4):424-427,456. |
| [3] | NI Dejiang,CHEN Yuqiong,XIE Bijun,et al.Studies on Composition Antioxidation and Hypoglyeemic Effects of Polysaccharides from Green Tea,Olong Tea and Black Tea[J].Acta Nutr Sin,2004,26(1):57-60(in Chinese).倪德江,陈玉琼,谢笔钧,等.绿茶、乌龙茶、红茶的茶多糖组成、抗氧化及降血糖作用研究[J].营养学报,2004,26(1):57-60. |
| [4] | CHEN Xiaoqiang,YE Yang,CHENG Hao,et al.Spectral Analysis of Crude Tea Polysaccharides Extracted by Different Methords[J].Spectrosc Spectr Anal,2009,29(4):1083-1087.(in Chinese).陈小强,叶阳,成浩等.不同方法提制的茶叶粗多糖的光谱分析[J].光谱学与光谱分析,2009,29(4):1083-1087. |
| [5] | FU Boqiang,XIE Mingyong,ZHOU Peng,et al.The Extraction of Tea Polysaecharide by Cellulase Degradation[J].J Wuxi Univ Light Ind,2002,21(4):362-366(in Chinese).傅博强,谢明勇,周鹏,等.纤维素酶法提取茶多糖[J].无锡轻工业大学学报,2002,21(4):362-366. |
| [6] | HUANG Yongchun,MA Yuefei,XIE Qingrou,et al.Study on Effects of Ultrasonic on Extracion of Tea Polysaccharide and Changes of Molecular Weight[J].Food Sci,2007,28(7):170-173(in Chinese).黄永春,马月飞,谢清若,等.超声波辅助提取茶多糖及其分子量变化的研究[J].食品科学,2007,28(7):170-173. |
| [7] | FU Boqiang,XIE Mingyong,ZHOU Peng.A Survey of The Studies on the Extraction,Purification,Composition and Pharmaceutical Effects of Tea Polysaceharide[J].J Nanchang Univ(Nat Sci),2001,25(4):358-364(in Chinese).傅博强,谢明勇,周鹏.茶叶多糖的提取纯化、组成及药理作用研究进展[J].南昌大学学报(理科版),2001,25(4):358-364. |
| [8] | CHEN Xiaoqiang,CHENG Hao,YE Yang,et al.Physicochemical Analysis of Purified Constituents of Tea Polysaccharides[J].Chinese Trad Herb Drugs,2008,39(6):828-830.陈小强,成浩,叶阳,等.茶多糖纯化组分的理化分析[J].中草药,2008,39(6):828-830. |
| [9] | Chen X Q,Lin Z,Ye Y,et al.Suppression of Diabetes in Non-Obese Diabetic(NOD)Mice by Oral Administration of Water-Soluble and Alkali-Soluble Polysaecharide Conjugates Prepared from Green Tea[J].Carbohydr Potym,2010,82(1):28-33. |
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