将二氧化锆(Zro2)分散于壳聚糖(CS)中得到稳定的CS-ZrO2复合物,制备了Hb/nano-Au/CS-ZrO2/Au过氧化氢生物传感器.用循环伏安法和计时电流法考察该修饰电极的电化学特性,发现该修饰电极对过氧化氢(H2O2)的还原有良好的电催化作用.实验结果表明,该传感器对H2O2的线性响应范围为9×10-6～1.8×10-3mol/L,线性相关系数R=0.9956,检测下限为5.6×10-7mol/L(S/N=3 ),并具有选择性高、线性范围宽和响应快等优点.
参考文献
| [1] | Zhang C,Zhang Z Y,Yu B B,et al.Application of the Biological Conjugate Between Antibody and Colloid Au Nanoparticles as Analyte to Inductively Coupled Plasma Mass Spectrometry[J].Anal Chem,2002,74 (1);96-99. |
| [2] | Thanh N T K,Kosenzweig Z.Development of An Aggregation-Based Immunoassay for Anti-Protein a using Gold Nanoparticles[J].Anal Chem,2002,74 (7) ;1624-1628. |
| [3] | Lei C X,Gong F C,Shen G L,et al.Amperometric Immunosensor for Schistosoma Japonicum Antigen using Antibodies Loaded on a Nano-Au Monolayer Modified Chitosan-Entrapped Carbon Paste Electrode[J].Sens Actuators B,2003,96:582-588. |
| [4] | Wu B Y,Hou S H,Yin F,et al.Amperometric Glucose Biosensor Based on Layer-by-Layer Assembly of Multilayer Films Composed of Chotosan Gold Nanoparticles and Glucose Oxidase Modified Pt Electrode[J].Biosens Bioelectron,2007,22:838-844. |
| [5] | Dobson K D,McQuillan A J.An Infrared Spectroscopic Study of Carbonate Adsorption to Zirconium Dioxide Sol-Gel Films from Aqueous Solutions[J].Langmuir,1997,13 (13):3392-3396. |
| [6] | Tong Z Q,Yuan R,Chai Y Q,et al.A Novel and Simple Biomolecules Immobilization Method:Electro-Deposition ZrO2 Doped with HRP for Fabrication of Hydrogen Peroxide Biosensor[J].J Biotechnol,2007,128 c 567-575. |
| [7] | Xu Y X,Hu C G,Hu S S.A Hydrogen Peroxide Biosensor Based on Direct Electrochemistry of Hemoglobin in Hb-Ag Sol Films[J].Sens Actuators B,2008,130:816-822. |
| [8] | Hong J M,Dai Z H.A Mperometric Biosensor for Hydrogen Peroxide and Nitrite Based on Hemoglobin Immobilized on One-Dimensional Gold Nanoparticle[J].Sens Actuators B,2009,140:222-226. |
| [9] | Liu S Q,Xu J J,Chen H Y.ZrO2 Gei-Derived DNA-modified Electrode and the Effect of Lanthanide on Its Electron Transfer Behavior[J].Bioelectrochemistry,2002,57 ;149-154. |
| [10] | Tang D P,Yuan R,Chai Y Q.Novel Potentiometric Immunosensor for the Detection of Diphtheria Antigen Based on Colloidal Gold and Polyvinyl Butyral as Matrixes[j].J Biochern Eng,2004,22 (1):45-49. |
| [11] | Wang M J,Wang L Y,Yuan H,et al.Immunosensors Based on Layer-by-Layer Self-Assembled Au Colloidal Electrode for the Electrochemical Detection of Antigen[j].Electroanalysis,2004,9:757-764. |
| [12] | Fang M,Kaschak D M,Sutorik A C,et al.A"Mix and Match" Ionic-Covalent Strategy for Self-Assembly of Inorganic Multilayer Films[J].J Am Chem Soc,1997,119:12184-12191. |
| [13] | Vanderkam S R,Gawalt E S,Schwartz J,et al.Electrochemically Active Surface Zirconium Complexes on Indium Tin Oxide[J].Langmuir,1999,15:6598-6600. |
| [14] | Pessoa C A,Gushikem Y,Kubota L T.Electrochemical Study of Methylene Blue Immobilized in Zirconium Phosphate[J].Electroanalysis,1997,9:800-803. |
| [15] | Yang F,Ruan C,Xu J,et al.An Amperometric Biosensor using Toluidine Blue as an Electron Transfer Mediator Intercalated in a-Zirconium Phosphate-Modified Horseradish Peroxidase Immobilization Matrix[J].Anal Chem,1998,361:115-118. |
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