制备了壳聚糖-g-N-羧甲基-二(2-苯并咪唑)-1,2-乙二醇(CTS-g-N-CBBIE),将其与纯化的纳米金溶胶(NGS)共混得到CTS-g-N-CBBIE-NGS复合物.以此复合物作为固酶载体固定云芝漆酶,固酶量大(31.10 mg/g),固酶比活力高(1.43 U/mg);此固酶复合物修饰的玻碳电极在无氧磷酸盐-柠檬酸盐缓冲溶液(pH=5.0)中可以实现无中介酶-电极直接电子迁移(一对准可逆氧化还原峰式电位576 mv(vs.Ag/AgCl)对应于漆酶活性中心T1位的氧化还原),电子迁移速率常数为228.3 s-1.当氧气浓度较小时,这种固酶修饰电极对氧气还原具有一定的生物电催化性能(空气饱和缓冲溶液中氧还原峰电位约为320mv(vs.Ag/AgCl)).当氧气浓度增高后,氧还原反应受到抑制;但这种漆酶修饰电极对pH较为敏感,且稳定性和重复使用性欠佳.
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