以缩水甘油基三甲基氯化铵(GTMA)、缩水甘油基三乙基氯化铵(GTEA)、缩水甘油基三丙基氯化铵(GTPA)、缩水甘油基三丁基氯化铵(GTBA)和缩水甘油基二甲基苄基氯化铵(GDMBA)等活性季铵盐为季铵化试剂,对N,O-2-羧乙基壳聚糖(N,O-2-CEC)进行了化学改性,得到了系列季铵化N,O-2-CEC(QCECs)；用电位滴定法测定了QCECs的季铵化度(DQ),分光光度法测定了N,O-2-CEC和QCECs的等电点(pHiep)；用琼脂平板法测定了N,O-2-CEC及其季铵化衍生物对金黄色葡萄球菌(S.aureus)和大肠杆菌(E.coli的最低抑菌浓度(MIC值),并根据N,O-2-CEC及其季铵化衍生物对这2种微生物的MIC值评价了它们的抗菌活性.结果表明,实验条件下得到的QCECs的DQ值在51.3％ ～59.1％间；N,O-2-CEC及其季铵化产物的等电点在6.44～7.30间,且季铵基团的引入会导致pHiep增大；除GTMA改性N,O-2-CEC的产物外,其它QCECs的pHiep随其季铵化度提高都有所增大；N,O-2-CEC和QCECs对S.aureus和E.coli均具有抗菌活性,且QCECs的MIC值均低于N,O-2-CEC的MIC值；同时,季铵基团中烃基碳数的增加及二甲基苄基结构的存在可提高QCECs的抑菌能力.
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