采用分光光度法研究了氨苄西林钠与茚三酮间的显色反应.确定了反应条件,建立了一种快速、简便测定氨苄西林钠的分光光度法.实验表明,氨苄西林钠与茚三酮在pH =5.4的水溶液中,沸水浴1h可以反应完全,产物在λm.=564 nm下,表观摩尔吸光系数为3.72×103 L/(mol·cm).氨苄西林钠质量浓度在10～ 90 mg/L范围内服从比耳定律,相关系数为0.9981,当氨苄西林钠浓度为40 mg/L时,测定结果的相对标准偏差为1.5％(n=6),回收率在99.0％以上.
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