采用气相色谱-质谱联用技术分析了北苍术和茅苍术的挥发油成分,分别鉴定出47和50种化学成分,并测定了其相对含量.采用色谱指纹图谱八强峰法和分区法,根据色谱峰的保留时间把总离子流色谱图分为5个区,比较了北苍术和茅苍术的挥发油成分.结果表明,在总离子流色谱图Ⅳ和Ⅴ区中,从北苍术和茅苍术中均检出苍术的主要药效成分β-桉叶油醇、苍术酮和苍术素,且相对含量较高.这表明《中国药典》将北苍术、茅苍术统称为苍术具有一定的科学性.
参考文献
| [1] | Editorial Committee of the Pharmacopoeia of the People's Republic of China.Pharmacopoeia of the People's Republic of China(Vol.1,2010)[S].Beijing:Chemical Industry Press,2010(in Chinese).国家药典委员会.中华人民共和国药典(一部2010年版)[S].北京:化学工业出版社,2010. |
| [2] | YAO Faye,LIU Wenying,QIU Qin.GC-MS Analysis of Volatile Oil from Atractylodes Chinensis[J].J Chinese Med Mater,2001,24(9):643-645(in Chinese).姚发业,刘文英,邱琴.北苍术挥发油的GC-MS分析[J].中药材,2001,24(9):643-645. |
| [3] | LI Yingchun,ZENG Jianqing,LIU Limei,et al.GC-MS Determination of Supercritical Extraction Products from the Rhizome ofAtractylodes Chinensis(DC.) Koidz.[J].J Instrum Anal,2001,20(1):46-48(in Chinese).李迎春,曾健青,刘莉玫,等.北苍术超临界CO2萃取产物的成分[J].分析测试学报,2001,20(1):46-48. |
| [4] | LI Xilin,XU Liyin,LUAN Jing.Extraction and Component Analysis of Volatile Oil from Atractylodes Chinensis[J].Acta Univ Trad Med Sinensis Pharmacol Shanghai,2008,22(1):59-61 (in Chinese).李西林,须丽茵,栾晶.北苍术挥发油的提取与成分分析[J].上海中医药大学学报,2008,22(1):59-61. |
| [5] | WU Zijing,DU Yuezhong,LIU Jingang,et al.Study on the Components of Volatile Oil of Atractylodes chinensis Koidz.by GC-MS[J].Ginseng Res,2010,(3):13-15 (in Chinese).武子敬,杜跃中,刘金刚,等.北苍术挥发油成分GC-MS分析[J].人参研究,2010,(3):13-15. |
| [6] | JI Li,AO Ping,PAN Jiongguang,et al.GC-MS Analysis of Essential Oils from Rhizomes of Atractylodes lancea(Thunb.)DC.and A.chinensis (DC.) Koidz[J].China J Chinese Mater Med,2001,26 (3):182-185 (in Chinese).吉力,敖平,潘炯光,等.苍术挥发油的气相色谱-质谱联用分析[J].中国中药杂志,2001,26(3):182-185. |
| [7] | Guo F Q,Huang L F,Zhou S Y,et al.Comparison of the Volatile Compounds of Atractylodes Medicinal Plants by Headspace Solid-phase Microextraction-Gas Chromatography-Mass Spectrometry[J].Anal Chim Acta,2006,570(1):73-78. |
| [8] | MENG Qing,FENG Yifan,GUO Xiaoling,et al.Studies on Chemical Constituents of Useful Parts from Atractylodes lancea (Thumb.) DC.[J].Chinese Trad Herb Drugs,2004,35(2):140-141 (in Chinese).孟青,冯毅凡,郭晓玲,等.苍术有效部位化学成分的研究[J].中草药,2004,35(2):140-141. |
| [9] | Li N,Deng C H,Li Y,et al.Gas Chromatography-Mass Spectrometry Following Microwave Distillation and Headspace Solid-phase Microextraction for Fast Analysis of Essential Oil in Dry Traditional Chinese Medicine[J].J Chromatogr A,2006,1133(1/2):29-34. |
| [10] | Chen Q H,Li P,Yang H D,et al.Identification of Volatile Compounds of Atractylode lancea Rhizoma Using Supercritical Fluid Extraction and GC-MS[J].J Sep Sci,2009,32(18):3152-3156. |
| [11] | WANG Liuying,QIAN Shihui,DUAN Jinao,et al.Analysis of Volatile Substances in Atractylodes Lancea[J].Lishizhen Med Mater Med Res,2009,20 (4):924-925 (in Chinese).汪六英,钱士辉,段金廒,等.苍术类药材挥发性成分的分析与比较[J].时珍国医国药,2009,20 (4):924-925. |
| [12] | ZHENG Huzhan,DONG Zehong,YU Jing.Modern Study and Application of Chinese Materia Medica[M].Beijing:Xueyuan Press,1997(in Chinese).郑虎占,董泽宏,余靖.中药现代研究与应用[M].北京:学苑出版社,1997. |
| [13] | Heller S R.EPA/NIH Mass Spectral Data Base[M].Washington:Government Printing Office,1980:45-50. |
| [14] | CONG Puzhu.Application of Mass Spectrum in Natural Organic Chemistry[M].Beijing:Science Press,1987:31-38 (in Chinese).丛浦珠.质谱学在天然有机化学中的应用[M].北京:科学出版社,1987:31-38. |
| [15] | Organic Chemistry Committee of Chinese Mass Spectrum Association. Collection of Illustrative Plates of Flavor Mass Spectrum[M].Beijing:Science Press,1992:76-78 (in Chinese).中国质谱学会有机专业委员会.香料质谱图集[M].北京:科学出版社,1992:76-78. |
| [16] | LI Yuhao,LIANG Songrning,SHAN Yuantiaoer. Anti-hypoxic Effect and Active Components of Atractylodes lancea (Thumb.) DC.[J].J Chinese Med Mater,1991,14(6):41-43(in Chinese).李育浩,梁颂名,山原(僚)二.苍术的抗缺氧作用及其活性成分[J].中药材,1991,14(6):41-43. |
| [17] | Li R L,Sakwiwatkul K,Li Y T,et al.Enhancement of the Immune Responses to Vaccination Against Foot-and-Mouth Disease in Mice by Oral Administration of an Extract Made from Rhizoma Atractylodis Macrocephalae(RAM)[J].Vaccine,2009,27(15):2094-2098. |
| [18] | Yoichiro N,Toshitaka K,Kazunori H,et al.Effect of the Rhizomes of Atractylodes Lancea and Its Constituents on the Delay of Gastric Emptying[J].J Ethnopharmacol,2003,84 (1):51-55. |
| [19] | ZENG Zhi,YANG Donghui,SONG Lifei,et al.The Identification of Isatis Indigotica Fort.by High Performance Liquid Chromatographic Fingerprint[J].Chinese J Anal Chem,2002,30 (7):849-852 (in Chinese).曾志,杨东晖,宋力飞,等.高效液相色谱指纹图谱应用于板蓝根的鉴定[J].分析化学,2002,30(7):849-852. |
| [20] | ZENG Zhi,ZHANG Yanping,LI He,et al.Application of Chromatograph-digitized Fingerprint in Quality Assessment of Forsythia Suspensa (thunb.) Vahl[J].Chinese Trad Pat Med,2005,27 (5):501-505 (in Chinese).曾志,张艳萍,李核,等.数字化色谱指纹图谱用于连翘的质量控制[J].中成药,2005,27(5):501-505. |
| [21] | ZENG Zhi,ZHANG Yanping,YANG Donghui,et al.Application of HPLC Fingerprint to Anemarrhena Asphodeloides[J].Chinese Trad Pat Med,2005,27 (10):1120-1124 (in Chinese).曾志,张艳萍,杨东晖,等.高效液相色谱指纹图谱在中药知母上的应用[J].中成药,2005,27(10):1120-1124. |
| [22] | ZENG Zhi,TAN Lixian,MENG Shaojin,et al.Study on the Chemical Constituents and Fingerprint of Pogostemon Cablin from Three Culture Varieties[J].Chinese J Anal Chem,2006,34 (9):1249-1254 (in Chinese).曾志,谭丽贤,蒙绍金,等.广藿香化学成分和指纹图谱研究[J].分析化学,2006,34(9):1249-1254. |
| [23] | TAN Lixian,ZENG Zhi,MENG Shaojin,et al.Study on the Volatile Constituents and Fingerprints of Centipeda Minima[J].J Instrum Anal,2006,25(6):91-94(in Chinese).谭丽贤,曾志,蒙绍金,等.石胡荽的挥发性成分和指纹图谱研究[J].分析测试学报,2006,25(6):91-94. |
| [24] | ZENG Zhi,XI Zhenchun,MENG Shaojin,et al.Study on Volatile Constitutions and Quality Evaluation of Different Varieties of Fructus Amomis[J].J Instrum Anal,2010,29 (7):701-706 (in Chinese).曾志,席振春,蒙绍金,等.不同品种砂仁挥发性成分及质量评价研究[J].分析测试学报,2010,29(7):701-706. |
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