建立了硝普钠分光光度法测定头孢他啶的方法.实验结果表明,在碱性条件下,头孢他啶与硝普钠以1∶2的化学计量比反应形成一种红褐色产物,其最大吸收波长为528 nm.头孢他啶在2.50～810 mg/L浓度范围内与吸光度呈良好线性关系,线性回归方程为A=0.02704+0.00218ρ (mg/L),线性相关系数r=0.9987,表观摩尔吸收系数ε528=1.2×103 L/(mol·cm),检测限(3σ/k)为1.38 mg/L.本方法操作简便、快速,成功用于药品中头孢他啶含量的测定.
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