采用混合菌BODseed制备微生物传感器快速监测生化需氧量(BOD),研究了矫正溶液选择、样品自降解及恒温装置对测量准确度的影响.结果表明,采用葡萄糖-谷氨酸矫正溶液测得污水处理厂污水结果较BOD5方法偏低50％以上,而以污水本身制作矫正曲线取得了与BOD5一致的测试结果；样品在保存6h后有机物自降解会使测量结果偏低40％；快速BOD在线监测时不仅需要对微生物传感器恒温,同时也需要对样品及缓冲溶液恒温,这有利于样品快速接近缓冲溶液温度,减少空气饱和时间,从而提高监测效率,提升测试准确度.
参考文献
| [1] | American Public Health Association Standard Methods for the Examination of Water and Wastewater[M]. 19th Ed.Washington,DC:American Public Health Association,1997. |
| [2] | Karube I,Matstnaga T,Mitsuda S,et al.A Microbial Electrode BOD Sensors[J].Biotechnol Bioeng,1977,19(10):1535-1547. |
| [3] | D'Souza S F.Microbial Biosensors[J].Biosens Bioelectron,2001,16(6):337-353. |
| [4] | JIA Jianbo,DONG Shaojun.The Progress of Microbial Biosensors for Biochemical Oxygen Demand[J].Chinese J Anal Chem,2003,31(6):742-748(in Chinese).郏建波,董绍俊.生化需氧量微生物传感器的研究进展[J].分析化学,2003,31(6):742-748. |
| [5] | Liu J,Mattiasson B.Microbial BOD Sensors for Wastewater Analysis[J].Water Res,2002,36(15):3786-3802. |
| [6] | Nomura Y,Chee G J,Karube I.Biosensor Technology for Determination of BOD[J].Field Anal Chem Technol,1998,2(6):333-340. |
| [7] | Praet E,Reuter V,Gaillard T,et al.Bioreactors and Biomembranes for Biochemical Oxygen Demand Estimation[J].Trends Anal Chem,1995,14(7):371-378. |
| [8] | Liu J,Bj(o)rnsson L,Mattiasosn B.Immobilised Activated Sludge Based Biosensor for BOD Measurement[J].Biosens Bioelectron,2000,14 (12):883-893. |
| [9] | Tan T C,Lim E W C.Thermally Killed Cells of Complex Microbial Culture for Biosensor Measurement of BOD of Wastewater[J].Sens Actuators B,2005,107(2):546-551. |
| [10] | DONG Shaojun,LI Bin,WANG Bingquan.Fabrication of Sensors Based on Sol-gel Organic-inorganic Hybrid Material:CN,97117266.8[P].1999-03-24(in Chinese).董绍俊,李彬,王炳全,溶胶-凝胶包埋酶制备生物传感器的方法:中国,97117266.8[P].1999-03-24. |
| [11] | LI Bin,KOU Wenpeng,DONG Shaojun. Grafting Copolymer of Poly (vinylalcohol) and 4-Vinylpyridine Hydrogel Synthesize:CN,96123527.6[P].1998-07-01 (in Chinese).李彬,寇文朋,董绍俊.聚乙烯醇桂枝4-乙烯基吡啶的制备方法:中国,96123527.6[P].1998-07-01. |
| [12] | Lehmann M,Chan C,Lo A,et al.Measurement of Biodegradable Substances Using the Salt-tolerant Yeast Arxula Adeninivorans for a Microbial Sensor Immobilized with Poly(carbamoyl) sulfonate(PCS) Part Ⅱ:Application of the Novel Biosensor to Real Samples from Coastal and Island Regions[J].Biosens Bioelectron,1999,14(3):295-302. |
| [13] | LIU Changyu,JIA Jianbo,DONG Shaojun. A Biosensor Type Biochemical Oxygen Demand Online Analyzer:CN,101000541B[P].2011-09-28(in Chinese).刘长宇,郏建波,董绍俊.一种传感器式生化需氧量在线监测仪:中国,101000341B[P]. 2011-09-28. |
上一张
下一张
上一张
下一张
计量
- 下载量()
- 访问量()
文章评分
- 您的评分：
-
10%
-
20%
-
30%
-
40%
-
50%