室温下一步合成了一种蓝色发光金量子点.该金量子点具有良好的水溶性和生物相容性,金量子点平均粒径为3.0nm,在波长305 nm光激发下,发射430 nm蓝色荧光.实验发现,一定量L-半胱氨酸对金量子点430 nm处荧光发射有显著的增强作用,由此可建立一种简单、迅速、灵敏检测L-半胱氨酸的新方法.在最佳条件下,金量子点荧光强度与L-半胱氨酸在0～4.0 μmol/L浓度范围内呈线性关系,线性相关系数R2=0.9976,对2.0μmol/L L-半胱氨酸的11次测定结果的相对标准偏差(RSD)为2.8％,以3倍标准偏差计算本法对L-半胱氨酸测定的检出限为5 nmol/L.
参考文献
| [1] | LI Ailing,GAO Lanxing.Effects of Amino Acids on Cardiovascular Function[J].Amino Acids Biotic Resour,1998,20 (2):45-49(in Chinese).李爱玲,高兰兴.氨基酸对心血管功能的影响[J].氨基酸和生物资源,1998,20(2):45-49. |
| [2] | XU Qishou.Progress on Amino Acid Pharmacology Research[J].Amino Acids Biotic Resour,1996,18(1):30-32(in Chinese).徐琪寿.氨基酸的药理学研究进展[J].氨基酸和生物资源,1996,18(1):30-32. |
| [3] | Richard-Forget F C,Goupy P M,Nicolas J J.Cysteine as an Inhibitor of Enzymic Browning:2.Kinetic Studies[J].J Agric Food Chem,1992,40(11):2108-2113. |
| [4] | SHAO Wei,MI Guangtai,WANG Chunxiang,et al.Studies on Effects of Cysteine on Stability of Ascorbic Acid[J].Chinese J Biochem Pharm,1995,16(5):219-227(in Chinese).邵伟,米广太,王春香,等.半胱氨酸对抗坏血酸稳定性影响的研究[J].中国生化药物杂志,1995,16(5):219-227. |
| [5] | DU Baozhong,YAO Binghua,XUE Li,et al.Determination of Cysteine and Cysteine with Potentiometric Titration[J].J Anal Sci,2004,20(6):622-624(in Chinese).杜宝中,姚秉华,薛力,等.电位滴定法测定半胱氨酸和胱氨酸的研究及应用[J].分析科学学报,2004,20(6):622-624. |
| [6] | Eid M A.Spectrophotometric Determination of Cysteine and N-Acetylcysteine in Pharmaceutical Preparations[J].Microchim Acta,1998,129(1):91-95. |
| [7] | LI Huaina,CI Yunxiang.Study on Chemiluminescence Reaction of Mercapto Compound-Cerium (Ⅳ)-Hydrocortisone by Flow Injection Analysis Chinese[J].J Anal Chem,1997,25(6):679-682 (in Chinese).李怀娜,慈云祥.用流动注射法研究巯基化合物-铈(Ⅳ)-氢化可的松的化学发光反应[J].分析化学,1997,25(6):679-682. |
| [8] | Lunar M L,Rubio S,Pérez-Bendito D,et al.Hexadecylpyridinium Chloride Micelles for the Simultaneous Kinetic Determination of Cysteine and Cystine by Their Induction of the Iodine-Azide Reaction[J].Anal Chim Acta,1997,337(3):341-349. |
| [9] | Li Y Z,Liu H F,Chang W B,et al.Selective Determination of Cytosine Based on Its Fluorogenic Reaction with Triethylamine/Chloroacetonitrile[J].Anal Lett,1998,31 (9):1499-1507. |
| [10] | HE Zhichan,LI Ruohua.Determination of Cysteine and Reducing Glutathione by Fluoressence Spectrophotometry[J].Technol Dev Chem Ind,2003,23(4):25-27(in Chinese).何志婵,李若华.荧光试剂N-[对-(2-苯并(嚼)唑基)-苯基]-马来酰亚胺测定半胱氨酸、谷胱甘肽的研究[J].化工技术与开发,2003,23(4):25-27. |
| [11] | Link S,Beeby A,Fitzgerald S,et al.Visible to Infrared Luminescence from a 28-Atom Gold Cluster[J].J Phys Chem B,2002,106(13):3410-3415. |
| [12] | Huang T,Murray R W.Visible Luminescence of Water-Soluble Monolayer-Protected Gold Clusters[J].J Phys Chem B,2001,105 (6):12498-12502. |
| [13] | Wilcoxon J P,Martin J E,Parsapour F,et al.Photoluminescence from Nanosize Gold Clusters[J].J Phys Chem,1998,108(21):9137-9142. |
| [14] | Zheng J,Petty J T,Dickson R M.High Quantum Yield Blue Emission from Water-Soluble Au8 Nanodots[J].J Am Chem Soc,2003,125(26):7780-7781. |
| [15] | Hwang Y N,Jeong D H,Shin H J,et al.Femtosecond Emission Studies on Gold Nanoparticles[J].J Phys Chem B,2002,106(31):7581-7584. |
| [16] | Dhara S,Chandra S,Magudapathy P,et al.Blue Luminescence of Au Nanoclusters Embedded in Silica Matrix[J].J Phys Chem,2004,121(24):12595-12599. |
| [17] | Shen X C,Jiang L F,Liang H,et al.Determination of 6-Mercaptopurine Based on The Fluorescence Enhancement of Au Nanoparticles[J].Talanta,69(2):456-462. |
| [18] | Huang C C,Yang Z S,Lee K H,et al.Synthesis of Highly Fluorescent Gold Nanoparticles for Sensing Mercury (Ⅱ)[J].Angew Chem Int Ed,2007,46(36):6824-6828. |
| [19] | Huang C C,Chiang C K,Lin Z H,et al.Bioconjugated Gold Nanodots and Nanoparticles for Protein Assays Based on Photoluminescence Quenching[J].Anal Chem,2008,80 (5):1497-1504. |
| [20] | Duan H,Nie S.Etching Colloidal Gold Nanocrystals with Hyperbranched and Multivalent Polymers:A New Route to Fluorescent and Water-Soluble Atomic Clusters[J].J Am Chem Soc,2007,129(9):2412-2413. |
| [21] | Habeeb Muhammed M,Ramesh S,Sinha S,et al,Two Distinct Fluorescent Quantum Clusters of Gold Starting from Metallic Nanoparticles by pH-Dependent Ligand Etching[J].Nano Res,2008,1 (4):333-340. |
| [22] | Shang L,Yin J,Li J,et al.Gold Nanoparticle-based Near-Infrared Fluorescent Detection of Biological Thiols in Human Plasma[J].Biosens Bioelectron,2009,25(2):269-274. |
| [23] | Shang L,Dong S.Sensitive Detection of Cysteine Based on Fluorescent Silver Clusters[J].Biosens Bioelectron,2009,24(6):1569-1573. |
| [24] | Han B,Wang E.Oligonucleotide-Stabilized Fluorescent Silver Nanoclusters for Sensitive Detection of Biothiols in Biological Fluids[J].Biosens Bioelectron,2011,26(5):2585-2589. |
| [25] | Chen X,Ko S K,Kim M J,et al.A Thiol-Specific Fluorescent Probe and Its Application for Bioimaging[J].Chem Commun,2010,46(16):2751-2753. |
| [26] | Han B,Yuan J,Wang E.Sensitive and Selective Sensor for Biothiols in the Cell Based on the Recovered Fluorescence of the CdTe Quantum Dots-Hg(Ⅱ) System[J].Anal Chem,2009,81(13):5569-5573. |
| [27] | Zhang Y,Li Y,Yan X P.Photoactivated CdTe/CdSe Quantum Dots as a Near Infrared Fluorescent Probe for Detecting Biothiols in Biological Fluids[J].Anal Chem,2009,81 (12):5001-5007. |
上一张
下一张
上一张
下一张
计量
- 下载量()
- 访问量()
文章评分
- 您的评分：
-
10%
-
20%
-
30%
-
40%
-
50%