测定了自制的α-1,2,3-K6H[SiW9V3O40]、α-1,2-K6[SiW10V2O40]和α-K5[SiW11VO40](以下分别简写为α-SiW9V3 、α-SiW10V2和α-SiW11V)钒取代多金属氧酸盐对蘑菇酪氨酸酶活性的抑制作用.结果表明,在pH =6.8的NaH2PO4-Na2HPO4缓冲溶液中,α-SiW9V3对酪氨酸酶有较强的抑制作用,表现为对酶稳态活力的抑制作用和对迟滞时间的延长作用,在DMSO溶液中,其IC50为0.6841 mmol/L,0.7 mmol/L α-SiW9V3可使单酚酶的迟滞时间由235 s延长至650 s,增加了2.77倍.α-SiW9V3对酪氨酸酶单酚酶的抑制为可逆作用过程,抑制类型为混合型,其抑制常数K1、KIS分别为4.22和2.39 mmol/L.α-SiW10V2不溶于DMSO,故未研究其对酶的抑制作用,α-SiW11V对酪氨酸酶抑制作用很弱.
参考文献
| [1] | Yi W,Dubois C,Yahiaoui S,et al.Refinement of Arylthiosemicarbazone Pharmacophore in Inhibition of Mushroom Tyrosinase[J].Eur J Med Chem,2011,46(9):4330-4335. |
| [2] | Yan Q,Cao R H,Yi W,et al.Inhibitory Effects of 5-Benzylidene Barbiturate Derivatives on Mushroom Tyrosinase and Their Antibacterial Activities[J].Eur J Med Chem,2009,44(10):4235-4243. |
| [3] | Fang Y T,Chen Y Z,Feng G F,et al.Benzyl Benzoates:New Phlorizin Analogs as Mushroom Tyrosinase Inhibitors[J].Bioorg Med Chem,2011,19(3):1167-1171. |
| [4] | Ha Y M,Kim J A,Park Y J,et al.Analogs of 5-(Substituted benzylidene)hydantoin as Inhibitors of Tyrosinase and Melanin Formation[J].BBA,2011,1810(6):612-619. |
| [5] | Li Z C,Chen L H,Yu X J,et al.Inhibition Kinetics of Chlorobenzaldehyde Thiosemicarbazones on Mushroom Tyrosinase[J].J Agric Food Chem,2010,58(23):12537-12540. |
| [6] | Takahashi S,Kamiya T,Saeki K,et al.Structural Insights into the Hot Spot Amino Acid Residues of Mushroom Tyrosinase for the Bindings of Thujaplicins[J].Bioorg Med Chem,2010,18(22):8112-8118. |
| [7] | Zhang J P,Chen Q X,Song K K,et al.Inhibitory Effects of Salicylic Acid Family Compounds on the Diphenolase Activity of Mushroom Tyrosinase[J].Food Chem,2006,95(4):579-584. |
| [8] | Yi W,Cao R H,Wen H,et al.Synthesis and Biological Evaluation of Helicid Analogues as Mushroom Tyrosinase Inhibitors[J].Bioorg Med Chem Lett,2008,18(24):6490-6493. |
| [9] | SONG Kangkang,QIU Ling,CHEN Qingxi.The Inhibitory Effect of Tyrosinase by Arbutin as Cosmetic Additive[J].J Xiamen Univ,2003,42(6):791-794(in Chinese).宋康康,丘凌,陈清西.熊果甙作为化妆品添加剂对酪氨酸酶抑制作用[J].厦门大学学报,2003,42(6):791-794. |
| [10] | ZOU Xianwei,JIANG Zhisheng.Advances in Studies on Botanical Inhibitors of Tyrosinase[J].Chinese Trad Herb Drugs,2004,35(6):702-705(in Chinese).邹先伟,蒋志胜.植物源酪氨酸酶抑制剂研究进展[J].中草药,2004,35(6):702-705. |
| [11] | Jeffrey T R,Craig L H,Deborah A J.Polyoxometalates in Medicine[J].Chem Rev,1998,98 (1):327-357. |
| [12] | Geng J,Li M,Qu X G,et al.Polyoxometalates as Inhibitors of the Aggregation of Amyloid β Peptides Associated with Alzheimer's Disease[J].Angew Chem Int Ed,2011,50(18):4184-4188. |
| [13] | XU Lin,WANG Enbo,HU Changwen.Assembly Syntheses of New Functional Molecule Based Materials Using Polyoxometalates as Building Blocks[J].Chinese J Inorg Chem,2000,16 (2):219-228 (in Chinese).许林,王恩波,胡长文.多金属氧酸盐作为构筑块自组装合成新型功能性分子材料[J].无机化学学报,2000,16(2):219-228. |
| [14] | Fraqueza G,Ohlin C A,Aureliano M,et al.Sarcoplasmic Reticulum Calcium ATPase Interactions with Decaniobate,Decavanadate,Vanadate,Tungstate and Molybdate[J].J Inorg Biochem,2012,107(1):82-89. |
| [15] | Wu Q,Wang J,Zhang L,et al.Molecular Recognition of Basic Fibroblast Growth Factor by Polyoxometalates[J].Angew Chem,2005,117(26):4116-4120. |
| [16] | Li J P,Gerard E,Yoram S,et al.Antilipolytic Actions of Vanadate and Insulin in Rat Adipocytes Mediated by Distinctly Different Mechanisms[J].Endocrinology,1997,138 (6):2274-2279. |
| [17] | Tsiani E,Bogdanovic E,Sorisky A,et al.Tyrosine Phosphatase Inhibitors,Vanadate and Pervanadate,Stimulate Glucose Transport and GLUT Translocation in Muscle Cells by a Mechanism Independent of Phosphatidylinositol 3-Kinase and Protein Kinase C[J].Diabetes,1998,47(11):1676-1686. |
| [18] | Peter J Domaille.1-and 2-Dimensional Tungsten-183 and Vanadium-51 NMR Characterization of Isopolymetalates and Heteropolymetalates[J].J Am Chem Soc,1984,106(25):7677-7687. |
| [19] | Luo Z,Kogerler P,Cao R,et al.Synthesis,Structure,and Magnetism of a Polyoxometalate with Coordinatively Unsaturated d-Electron-Transition Metal Centers[J].Inorg Chem,2009,48(16):7812-7817. |
| [20] | CHEN Bingnian,FENG Zhenge,WANG Li.Antibacterial Activities of Polyoxometalates Containing Silicon[J].Chem J Chinese Univ,2011,32(5):1033-1036(in Chinese).陈丙年,冯珍鸽,王力.含硅多金属氧酸盐的抑菌作用[J].高等学校化学学报,2011,32(5):1033-1036. |
| [21] | YANG Yong,FENG Lin.Preparation,Characterization and Biological Activity of Organotitanium Substitut Heteropolytungstate with Keggin Structure[J].J Chongqing Normal Univ,2006,23(1):52-54(in Chinese).杨勇,冯琳.有机钛取代的Keggin型硅钨杂多化合物的合成、表征及其生物活性研究[J].重庆师范大学学报,2006,23(1):52-54. |
| [22] | WANG Rui.Synthesis of Silicotungstates and Their Catalytic Performance for Tetrahydropyranylation of Alcohol[J].Chem Res,2009,20(2):79-82(in Chinese).王锐.硅钨酸盐的合成及其在醇的四氢吡喃化反应中的催化性能[J].化学研究,2009,20(2):79-82. |
| [23] | LUO Qian,JIAN Min,ZHANG Jin,et al.Study on the Synthesis of Heteropoly Acids and Their Catalytic Performance for the Direct Hydroxylation of Benzene to Phenol[J].Chem Res Appl,2006,18 (6):629-633(in Chinese).罗茜,简敏,张进,等.硅钼钒和硅钨钒杂多酸的制备及其在苯直接羟基化制苯酚中的活性研究[J].化学研究与应用,2006,18 (6):629-633. |
| [24] | WANG Li,CAI Wei,OU Guangnan.Reconstructed Synthetic and Characterization of a Insoluble Polyoxometalate Coordination Polymer[J].ChemJ Chinese Univ,2009,30(11):2136-2138(in Chinese).王力,蔡薇,欧光男.一种难溶多金属氧酸盐配位聚合物的重构与表征[J].高等学校化学学报,2009,30(11):2136-2138. |
| [25] | GONG Shengzhao,DENG Xiangqing,LI Shimei.Kinetics of Tyrosinase Inhibition by 4-Hydroxycinnamic Acid[J].China Surfact Deterg Cosmet,2006,36(3):159-162(in Chinese).龚盛昭,邓相庆,李仕梅.对羟基肉桂酸抑制酪氨酸酶活性的动力学研究[J].日用化学工业,2006,36(3):159-162. |
| [26] | GONG Shengzhao,YANG Zhuoru,CHENG Jiang.Inhibition of Tyrosinase Activity by Vanillin[J].J South China Univ Technol,2006,34(5):53-57(in Chinese).龚盛昭,杨卓如,程江.香草醛对酪氨酸酶活性的抑制[J].华南理工大学学报,2006,34(5):53-57. |
| [27] | Chen Q X,Song K K,Qiu L,et al.Inhibitory Effects on Mushroom Tyrosinase by p-Alkoxybenzoic Acids[J].Food Chem,2005,91(2):269-274. |
| [28] | LIU Xiaodan,HUANG Huang,CHEN Qingxi.Studies on Inhibitory Effects of Benzoic Acid on Mushroom Tyrosinase[J].J Xiamen Univ,2003,42(1):102-106(in Chinese).刘晓丹,黄璜,陈清西.苯甲酸对蘑菇酪氨酸酶抑制作用机理的研究[J].厦门大学学报,2003,42(1):102-106. |
| [29] | Zhu Y J,Zhou H T,Hu Y H,et al.Antityrosinase and Antimicrobial Activities of 2-Phenylethanol,2-Phenylacetaldehyde and 2-Phenylacetic Acid[J].Food Chem,2011,124(1):298-302. |
| [30] | ZHU Xiangzhi,PAN Dongming.Comparison of the Inhibition of Some Ethanol Extracts from Different Kinds of Fructus Aurantii on the Activity of Mushroom Tyrasinase[J].J Xiamen Univ,2005,44(3):416-419(in Chinese).朱祥枝,潘东明.几种枳壳乙醇提取物对酪氨酸酶抑制作用比较[J].厦门大学学报,2005,44(3):416-419. |
| [31] | GUO Huayun,SONG Kangkang,CHEN Qingxi,et al.Studies on Inhibitory Effect of 5-Methoxysalicylaldehyde on Tyrosianse[J].J Xiamen Univ,2007,46(1):87-90(in Chinese).郭华云,宋康康,陈清西,等.5-甲氧基水杨醛对蘑菇酪氨酸酶的抑制作用[J].厦门大学学报,2007,46(1):87-90. |
| [32] | Aureliano M,Henao F,Crans D C,et al.Sarcoplasmic Reticulum Calcium ATPase is Inhibited by Organic Vanadium Coordination Compounds:Pyridine-2,6-dicarboxylatodioxovanadium(V),BMOV,and an Amavadine Analogue[J].Inorg Chem,2008,47 (13):5677-5684. |
| [33] | HUANG Huang,SONG Kangkang,CHEN Qingxi.Studies on Whitening Effect of Kojic Acid as Cosmetic Additive[J].J Xiamen Univ,2003,42 (5):652-656 (in Chinese).黄璜,宋康康,陈清西.曲酸作为化妆品添加剂的增白作用机理研究[J].厦门大学学报,2003,42 (5):652-656. |
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