制备了一种新型电化学传感器——聚2,2-联吡啶(Pbpy)/1-苯基-3-甲基4-(α-呋喃甲酰基)-5-吡唑啉酮(HPMαFP)/玻碳(GC)复合膜修饰电极.运用循环伏安法和脉冲伏安法研究了药物分子黄嘌呤(XN)的电化学行为及反应机理.与裸GCE和Pbpy/GCE电极相比,复合修饰电极Pbpy/HPMαFP/GCE测定XN的氧化峰电流和检测灵敏度均有显著提高,表明聚2,2-联吡啶与酰基吡唑啉酮产生了协同增效作用.当pH =8,扫速为100 mV/s的条件下,氧化峰电流与黄嘌呤浓度在6×10-7～1×10-5 mol/L和1×10-5～1 ×10-4 mol/L之间均呈现良好的线性关系,检出限为1×10-8 mol/L.该修饰电极可用于共存尿酸(UA)、抗坏血酸(AA)体系及实际样品的黄嘌呤含量测定.
参考文献
| [1] | Palraj K,John S A.Simultaneous Determination of Ascorbic Aacid,Dopamine,Uric Acid and Xanthine Using a Nanostructured Polymer Film Modified Electrode[J].Talanta,2010,80(5):1686-1691. |
| [2] | George G Guilbault.Analytical Uses of Immobilized Dnzymes[M].New York:Marcel Dekker,1984:453. |
| [3] | WANG Zhenhui,ZHANG Hui.An Inlail Super-thin Carbon Paste Film Modified Electrode for Simultaneous Determination of Xanthine and Hypoxanthine[J].Chinese J Anal Chem,2005,33 (5):671-674 (in Chinese).汪振辉,张卉.嵌入式超薄碳糊膜电极伏安法同时测定黄嘌呤和次黄嘌呤[J].分析化学,2005,33(5):671-674. |
| [4] | Palra K,John J S A.Simultaneous Determination of Epinephrine,Uric Acid and Xanthine in the Presence of Ascorbic Acid Using an Ultrathin Polymer Film of 5-Amino-1,3,4-thiadiazole-2-thiol Modified Electrode[J].Anal Chim Acta,2009,647:97-103. |
| [5] | Zen J M,Lai Y Y,Yang H H,et al.Multianalyte Sensor for the Simultaneous Determination of Hypoxanthine,Xanthine and Uric Acid Based on a Preanodized Nontronite-coated Screen-printed Electrode[J].Sens Actuat B-Chem,2002,84(2/3):237-244. |
| [6] | Sun D,Zhang Y,Wang F R,et al.Electrochemical Sensor for Simultaneous Detection of Ascorbic Acid,Uric Acid and Xanthine Based on the Surface Enhancement Effect of Mesoporous Silica[J].Sens Actuat B-Chem,2009,141(2):641-645. |
| [7] | David J Garrett,Peter Jenkins,Matthew I J.Diazonium Salt Derivatives of Osmium Bipyridine Complexes:Electrochemical Grafting and Characterisation of Modified Surfaces[J].Electrochim Acta,2011,56:2213-2220. |
| [8] | Shan D,Qian B,Ding S N,et al.Enhanced Solid-state Electrochemiluminescence of Tris(2,2'-bipyridyl) Ruthenium(Ⅱ)Incorporated into Electrospun Nano Fibrous Mat[J].Anal Chem,2010,82:5892-5896. |
| [9] | Hun X,Zhang Z J.Electrogenerated Chemiluminescence Sensor for Itopride with Ru (bpy) 23 +-doped Silica Nanoparticles/Chitosan Composite.Films Modified Electrode[J].Sens Actuat B,2008,131:403-410. |
| [10] | Ding S N,Xu J J,Zhang W J,et al.Tris(2,2-bipyridyl) Ruthenium(Ⅱ)-Zirconia-Nafion Composite Modified Electrode Applied as Solid-state Electrochemiluminescence Detector on Electrophoretic Microchip for Detection of Pharmaceuticals of Tramadol,Lidocaine and Ofloxacin[J].Talanta,2006,70:572-577. |
| [11] | XU Liying,LI Jinzhou,ZHENG Xiaodan,et al.A Study on Adsorptive Wave of Triple Complex of Furoyl Pyrazalone with Lead[J].Chinese J Inorg Chem,2005,21 (11):1614-1618 (in Chinese).徐丽英,李锦州,郑晓丹,等.呋喃甲酰基吡唑啉酮铅三元配合物吸附波的研究[J].无机化学学报,2005,21(11):1614-1618. |
| [12] | Ghoneim M M,El-Baradie K Y,Tawfik A.Electrochemical Behavior of the Antituberculosis Drug Isoniazid and Its Squarewave Adsorptive Stripping Voltammetric Estimation in Bulk Form,Tablets and Biological Fluids at a Mercury Electrode[J].J Pharm Biomed Anal,2003,33(4):673-685. |
| [13] | Zhang D,Li J Z.Acylpyrazolone Modified Glassy Carbon Electrode Effect on the Electrochemical Behavior of Tyrosine[J].Anal Lett,2008,41 (15):2832-2843. |
| [14] | Li X L,Li J Z.Direct Determination Guanine in Aciclovir Dispersible Tablets Solution by Acylpyrazolone Modified Glassy Carbon Electrod[J].Rev Anal Chem,2011,30(1):23-27. |
| [15] | SONG Yun,LI Jinzhou,LIU Yong.Determination of Isoniazid on Multi-Walled Carbon Nanotubes-HPMaFP Composite Film Modified Glassy Carbon Electrode[J].Chem Res Appl,2011,23 (5):578-582 (in Chinese).宋昀,李锦州,刘永.多壁碳纳米管-酰基吡唑啉酮复合膜修饰电极测定异烟肼[J].化学研究与应用,2011,23(5):578-582. |
| [16] | Song Y,Li J Z.Direct Electrochemical Determination of Admine Using HPMαFP-Modified Glassy Carbon Electrode in Commercial Pharmaceutical Products[J].Instrum Sci Technol,2011,39 (3):261-272. |
| [17] | DONG Xuechang,LIU Fuchu,ZHAO Yanlai.Synthesis of Some New Acyl Pyrazolone Type Chelating Agents[J].Acta Chim Sin,1983,41 (9):848-852 (in Chinese).董学畅,刘复初,赵雁来.几种新的4-酰基吡唑琳酮类螯合剂的合成[J].化学学报,1983,41(9):848-852. |
| [18] | Sun W,Li Y Z,Duan Y Y,et al.Direct Electrocatalytic Oxidation of Adenine and Guanine on Carbon Ionic Liquid Electrode and the Simultaneous Determination[J].Biosens Bioelectron,2008,24(4):988-993. |
| [19] | Anik U,Cubukcu M.Examination of the Electroanalytic Performance of Carbon Nanotube(CNT) Modified Carbon Paste Electrodes as Xanthine Biosensor Transducers[J].Turk J Chem,2008,32(6):711-719. |
| [20] | YANG Yunfa.Determination of Xanthine by Anodic Differential Pulse Voltammetry[J].Chinese J Anal Chem,1997,25 (11):1311-1314 (in Chinese).杨运发.阳极微分脉冲伏安法测定黄嘌呤[J].分析化学,1997,25(11):1311-1314. |
| [21] | Xiao F,Zhao F Q,Li J W,et al.Characterization of Hydrophobic Ionic Liquid-carbon Nanotubes Gold Nanoparticles Composite Film Coated Electrode and the Simultaneous Voltammetric Determination of Guanine and Adenine[J].Electrochim Acta,2008,53 (26):7781-7788. |
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