采用流动注射-石英晶体微天平(FIA-QCM)和紫外光谱法研究了牛/羊血清蛋白(BSA和GSA)与扁桃酸的手性识别.在QCM表面,BSA和GSA的浓度分别为4.9×10ˉ12和8.8×10ˉ12 mol/cm2.血清蛋白与R-扁桃酸(R-MA)作用引起QCM振动频率变化值比S-扁桃酸(S-MA)大；相应紫外结合常数也比与S-MA大,说明血清蛋白对R-MA的作用强于与S-对映体.从识别因子分析,GSA对扁桃酸的手性识别能力优于BSA.
参考文献
| [1] | Berthod A.Chiral Recognition Mechanisms[J].Anal Chem,2006,78(7):2093-2099. |
| [2] | Joseph K S,Moser A C,Basiaga S B G,et al.Evaluation of Alternatives to Warfarin as Probes for Sudlow Site I of Human Serum Albumin:Characterization by High-Performance Affinity Chromatography[J].J Chromatogr A,2009,1216 (16):3492-3500. |
| [3] | Cao C,Yin Y Y,Quan L,et al.Weak Affinity Ligands Selection Using Quartz Crystal Microbalance Biosensor:Multihydroxyl Amine Ligands for Protein Separation[J].Anal Chim Acta,2011,703(2):250-256. |
| [4] | Liu Y,Yu X,Zhao R,et al.Quartz Crystal Biosensor for Real-time Monitoring of Molecular Recognition Between Protein and Small Molecular Medicinal Agents[J].Biosens Bioelectron,2003,19(1):9-19. |
| [5] | Su W C,Zhang W G,Zhang S,et al.A Novel Strategy for Rapid Real-time Chiral Discrimination of Enantiomers Using Serum Albumin Functionalized QCM Biosensor[J].Biosens Bioelectron,2009,25 (2):488-492. |
| [6] | Chen W J,Zhang W G,Fan J,et al.A New Biosensor for Chiral Recognition Using Goat and Rabbit Serum Albumin SelfAssembled QCM[J].Chirality,2012,24(10):804-809. |
| [7] | Wang X Y,Ruengruglikit C,Wang Y W,et al.Interfacial Interactions of Pectin with Bovine Serum Albumin Studied by Quartz Crystal Microbalance with Dissipation Monitoring:Effect of Ionic Strength[J].J Agric Food Chem,2007,55(25):10425-10431. |
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