离子排斥色谱法同时测定葡萄糖醛酸和内酯及葡萄糖醛酸稳定性

应用化学, 2014, 31(4): 450-454. doi: 10.3724/SP.J.1095.2014.30299

离子排斥色谱法同时测定葡萄糖醛酸和内酯及葡萄糖醛酸稳定性

范艳群 ^1, , 许建中 ^2, , 徐询 ^3, , 刘亿婕 ^4, , 许晨 ^5,

1.厦门大学生命科学学院厦门 361005;国家海洋局第三海洋研究所厦门 361102

2.国家海洋局第三海洋研究所厦门 361102

3.厦门大学生命科学学院厦门 361005;国家海洋局第三海洋研究所厦门 361102

4.福建医科大学福州 350108

5.国家海洋局第三海洋研究所厦门 361102

基金项目: 国家海洋公益性行业专项(201005022) 中国大洋矿产资源研究开发协会专项(DY125-15-T-01) the China Ocean Mineral Resources R&D Association(DY125-15-T-01) the Ocean Public Welfare Scientific Research Project(201005022)

关键词：离子排斥色谱 , 葡萄糖醛酸 , 葡萄糖醛酸内酯 , 稳定性

Simultaneous Determination of Glucuronic Acid and Glucuronolactone Using Ion-exclusion Chromatography

Keywords： ion-exclusion chromatography , glucuronic acid , glucuronolactone , stability

建立了同时检测葡萄糖醛酸和葡萄糖醛酸内酯的离子排斥色谱法.采用Boston HC-B75 H+色谱柱进行分离,以5 mmol/L H2SO4水溶液为流动相,流速0.6 mL/min,柱温25℃,紫外检测波长220 nm;根据外标法定量,葡萄糖醛酸和内酯分别在10～1200 mg/L和5～600 mg/L范围内线性良好(r＞0.9999);葡萄糖醛酸、葡萄糖醛酸内酯的最低检测限及定量限均分别为0.1和0.3 mg/L,精密度实验RSD分别为1.00％和0.35％,平均回收率分别为99.69％和98.21％.该检测方法简便、快速、灵敏,可用于葡萄糖醛酸和葡萄糖醛酸内酯的同时测定.温度、pH值和溶剂对葡萄糖醛酸稳定性有影响,可为葡萄糖醛酸的分析与制备研究提供参考依据.

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