建立了一种采用离子液体1-己基-3-甲基咪唑六氟磷酸([C6mim][PF6])为萃取剂,超声辅助离子液体液相微萃取-高效液相色谱法分析水样中加替沙星和氟罗沙星的方法.实验考察了溶液酸度、离子液体用量等因素对萃取的影响.在pH值分别为3.3、2.1的加替沙星和氟罗沙星水样中,加入0.4mL[C6mim][PF6],超声,离心,离子液体相直接用于HPLC进行分析.该方法的线性范围为0.5～ 50 mg/L,测定加替沙星和氟罗沙星的相对标准偏差(n=5)为2.80％和5.93％,二者的检出限分别为0.46、0.97 μg/L,该方法萃取水样中加替沙星的加标回收率为80.5％～ 89.5％,氟罗沙星的加标回收率可达93.3％～99.0％.
参考文献
| [1] | ZHANG Yujie,LI Qian,WANG Xia,et al.Determination of Fluoroquinolones in Tissues of Several Animals by HPLC[J].Chinese J Vet Drug,2012,46(7):65-68(in Chinese).张玉洁,李倩,汪霞,等.高效液相色谱法检测多种动物组织中氟喹诺酮类药物残留量的研究[J].中国兽药杂志,2012,46(7):65-68. |
| [2] | YU Suhua,MENG Wenjing,LU Tingting,et al.Spectrophotometric,Spectrofluorimetric and Reversed-phase High-performance Liquid Chromatographic Methods for Determination of Gatifloxacin in Its Capsule[J].JYangzhou Univ (Nat Sci Edn),2008,11(2):40-44(in Chinese).于素华,孟文静,陆婷婷,等.加替沙星的紫外、荧光分光光度法和高效液相色谱法测定[J].扬州大学学报(自然科学版),2008,11(2):40-44. |
| [3] | LI Sheng,YAO Junxue,AODENG Gaowa.Spectrophot Ometric Study on Fleroxacin and Its Application[J].J Inner Mongolia Univ(Nat Sci Edn),2005,36 (3):273-275(in Chinese).李胜,姚俊学,敖登高娃.氟罗沙星的分光光度法研究及应用[J].内蒙古大学学报(自然科学版),2005,36(3):273-275. |
| [4] | Han J,Wang Y,Yu C L.Extraction and Determination of Chloramphenicol in Feed Water,Milk,and Honey Samples Using an Ionic Liquid/Sodium Citrate Aqueous Two-phase System Coupled with High-performance Liquid Chromatography[J].Anal Bioanal Chem,2011,399:1295-1304. |
| [5] | Zhang Y F,Lee H.Ionic Liquid-based Ultrasound-assisted Dispersive Liquid liquid Microextraction Followed High-performance Liquid Chromatography for the Determination of Ultraviolet Filters in Environmental Water Samples[J].Anal Chim Acta,2012,750:120-126. |
| [6] | Han D D,Row K H.Determination of Luteolin and Apigenin in Celery Using Ultrasonic-assisted Extraction Based on Aqueous Solution of Ionic Liquid Coupled with HPLC Quantification[J].J Sci Food Agric,2011,91:2888-2892. |
| [7] | Ma C H,Wang S Y,Yang L,et al.Ionic Liquid-aqueous Solution Ultrasonic-assisted Extraction of Camptothecin and 10-Hydroxycamptothecin from Camptotheca Acuminata Samara[J].Chem Eng Proc,2012,57:59-64. |
| [8] | Lou Z X,Wang H X,Zhu S,et al.Ionic Liquids Based Simultaneous Ultrasonic and Microwave Assisted Extraction of Phenolic Compounds from Burdock Leaves[J].Anal Chim Acta,2012,716:28-33. |
| [9] | LIU Meihua,QIU Bin,CHEN Guonan,et al.Determ Ination of Estrogens in Waste Water by Ultrasound-Assisted Ionic Liquid Dispersive Liquid-Phase Microextraction Coupled with High Performance Liquid Chromatography[J].Anal Test Technol Instrum,2009,15(3):151-157(in Chinese).刘美华,邱彬,陈国南,等.超声辅助离子液体分散液相微萃取-高效液相色谱法测定废水中雌激素的研究[J].分析测试技术与仪器,2009,15(3):151-157. |
| [10] | WANG Ying,XIAO Yao,REN Ruibing,et al.Homogeneous Ionic Liquid Microextraction of Fleroxacin and Sulfaquinoxaline from Water Samples[J].Chem J Chinese Univ,2010,9(31):1721-1724(in Chinese).王影,肖瑶,任瑞冰,等.水样中氟罗沙星和磺胺喹噁啉的离子液体均匀提取[J].高等学校化学学报,2010,9(31):1721-1724. |
| [11] | ChunS,Dzyuba S V,Bartsch R A.Influence of Structural Variation in Room-Temperature Ionic Liquids on the Selectivity and Efficiency of Competitive Alkali Metal Salt Extraction by a Crown Ether[J].Anal Chem,2001,73:3737-3741. |
| [12] | DU Liming,JIN Weijun,DONG Chuan,et al.Studies on Correlation Between Protonation and Tautomerism and Luminescence Characteristics of Quinolone[J].Spectrosc Spectr Anal,2001,21(4):518-520(in Chinese).杜黎明,晋卫军,董川,等.喹诺酮类药物的质子化作用及互变异构现象与荧光特性的相关性研究[J].光谱学与光谱分析,2001,21(4):518-520 |
| [13] | Visser A E,Swatloski R P,Reichert W M,et al.Traditional Extractions in Notraditional Solvents:Groups 1 and 2 Extraction by Crown Ethers in Room-temperature Ionic Liquids[J].Ind Eng Chem Res,2000,39:3596-3604. |
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