L-谷氨酸消旋化反应对于制备光学纯D-谷氨酸具有重要的意义.本文利用532 nm激光诱导荧光共聚焦检测式毛细管阵列电泳仪研究了L-谷氨酸基于席夫碱中间物的消旋化反应,采用羧基四甲基罗丹明琥珀酰亚胺酯(TAMRA)荧光探针标记消旋化产物后,以含有2 mmol/L 环糊精(β-CD)的100 mmol/L Tris-硼酸缓冲液(pH 10)为分离电解质进行毛细管阵列电泳分析,在该条件下TAMRA标记的谷氨酸对映体可以得到基线拆分.详细考察了醛的种类及用量、羧酸溶剂的种类、反应体系的含水量对消旋化的影响,结果表明L-谷氨酸在含20%(体积分数)水的乙酸溶剂中,以水杨醛作为催化剂(水杨醛与L-谷氨酸的物质的量比为0.2)的优化条件下可以快速地被消旋化.
L-Glutamic acid (Glu) racemization research is of great importance for the preparation of optical pure D-Glu.In the present work,we studied L-Glu racemization reaction through Schiff base intermediate by using capillary array electrophoresis (CAE) with 532 nm laser-induced confocal fluorescence detection.The racemization products were labeled by carboxytetramethylrhodamine succinimidyl ester (TAMRA) and analyzed in a 100 mmol/L Tris-borate buffer (pH 10) system containing 2 mmol/L β-CD by CAE.Under this condition,the TAMRA-Glu enantiomer can be completely separated.The influences of the type and dosage of the aldehyde,organic acids,as well as the water content in the reaction system on the racemization were examined in detail,and L-Glu was racemized quickly in the presence of 0.2 molar ratio of L-Glu to salicylaldehyde (as the catalyzer) in acetic acid containing 20%(v/v) water.
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